論文 - 詳細
| RRC ID | 27314 |
|---|---|
| 著者 | Koike S, Matsukura C, Takayama M, Asamizu E, Ezura H. |
| タイトル | Suppression of γ-aminobutyric acid (GABA) transaminases induces prominent GABA accumulation, dwarfism and infertility in the tomato (Solanum lycopersicum L.). |
| ジャーナル | Plant Cell Physiol |
| Abstract |
Tomatoes accumulate γ-aminobutyric acid (GABA) at high levels in the immature fruits. GABA is rapidly converted to succinate during fruit ripening through the activities of GABA transaminase (GABA-T) and succinate semialdehyde dehydrogenase (SSADH). Although three genes encoding GABA-T and both pyruvate- and α-ketoglutarate-dependent GABA-T activities have been detected in tomato fruits, the mechanism underlying the GABA-T-mediated conversion of GABA has not been fully understood. In this work, we conducted loss-of-function analyses utilizing RNA interference (RNAi) transgenic plants with suppressed pyruvate- and glyoxylate-dependent GABA-T gene expression to clarify which GABA-T isoforms are essential for its function. The RNAi plants with suppressed SlGABA-T gene expression, particularly SlGABA-T1, showed severe dwarfism and infertility. SlGABA-T1 expression was inversely associated with GABA levels in the fruit at the red ripe stage. The GABA contents in 35S::SlGABA-T1(RNAi) lines were 1.3-2.0 times and 6.8-9.2 times higher in mature green and red ripe fruits, respectively, than the contents in wild-type fruits. In addition, SlGABA-T1 expression was strongly suppressed in the GABA-accumulating lines. These results indicate that pyruvate- and glyoxylate-dependent GABA-T is the essential isoform for GABA metabolism in tomato plants and that GABA-T1 primarily contributes to GABA reduction in the ripening fruits. |
| 巻・号 | 54(5) |
| ページ | 793-807 |
| 公開日 | 2013-5-1 |
| DOI | 10.1093/pcp/pct035 |
| PII | pct035 |
| PMID | 23435575 |
| MeSH | 4-Aminobutyrate Transaminase / metabolism* Amino Acids / metabolism Flowers / growth & development Fruit / growth & development Gene Expression Regulation, Plant Genes, Plant / genetics Glutamic Acid / metabolism Lycopersicon esculentum / enzymology* Lycopersicon esculentum / genetics Lycopersicon esculentum / growth & development* Metabolic Networks and Pathways / genetics Plant Infertility* Plant Leaves / enzymology Plant Leaves / genetics Plant Stems / metabolism Plants, Genetically Modified RNA Interference Suppression, Genetic* gamma-Aminobutyric Acid / metabolism* |
| IF | 4.062 |
| 引用数 | 25 |
| WOS 分野 | PLANT SCIENCES CELL BIOLOGY |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 最多言及媒体 | Patent(IFI CLAIMS) |
| 各媒体での言及数の合計 | 1 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| トマト | TOMJPF00001 |