| RRC ID |
27329
|
| Author |
Kurokawa N, Hirai T, Takayama M, Hiwasa-Tanase K, Ezura H.
|
| Title |
An E8 promoter-HSP terminator cassette promotes the high-level accumulation of recombinant protein predominantly in transgenic tomato fruits: a case study of miraculin.
|
| Journal |
Plant Cell Rep
|
| Abstract |
KEY MESSAGE:The E8 promoter-HSP terminator expression cassette is a powerful tool for increasing the accumulation of recombinant protein in a ripening tomato fruit. Strong, tissue-specific transgene expression is a desirable feature in transgenic plants to allow the production of variable recombinant proteins. The expression vector is a key tool to control the expression level and site of transgene and recombinant protein expression in transgenic plants. The combination of the E8 promoter, a fruit-ripening specific promoter, and a heat shock protein (HSP) terminator, derived from heat shock protein 18.2 of Arabidopsis thaliana, produces the strong and fruit-specific accumulation of recombinant miraculin in transgenic tomato. Miraculin gene expression was driven by an E8 promoter and HSP terminator cassette (E8-MIR-HSP) in transgenic tomato plants, and the miraculin concentration was the highest in the ripening fruits, representing 30-630 μg miraculin of the gram fresh weight. The highest level of miraculin concentration among the transgenic tomato plant lines containing the E8-MIR-HSP cassette was approximately four times higher than those observed in a previous study using a constitutive 35S promoter and NOS terminator cassette (Hiwasa-Tanase et al. in Plant Cell Rep 30:113-124, 2011). These results demonstrate that the combination of the E8 promoter and HSP terminator cassette is a useful tool to increase markedly the accumulation of recombinant proteins in a ripening fruit-specific manner.
|
| Volume |
32(4)
|
| Pages |
529-36
|
| Published |
2013-4-1
|
| DOI |
10.1007/s00299-013-1384-7
|
| PMID |
23306632
|
| MeSH |
Arabidopsis
Arabidopsis Proteins / genetics
Fruit / genetics
Fruit / metabolism
Gene Expression Regulation, Plant
Glycoproteins / biosynthesis*
Glycoproteins / genetics
Heat-Shock Proteins / genetics
Lycopersicon esculentum / genetics*
Lycopersicon esculentum / metabolism
Plants, Genetically Modified / genetics
Plants, Genetically Modified / metabolism
Promoter Regions, Genetic*
Recombinant Proteins / biosynthesis*
Recombinant Proteins / genetics
Terminator Regions, Genetic*
|
| IF |
3.825
|
| Times Cited |
10
|
|
WOS Category
|
PLANT SCIENCES
|
| Resource |
| Tomato |
TOMJPF00001 |
