RRC ID 27332
Author Iwai H, Terao A, Satoh S.
Title Changes in distribution of cell wall polysaccharides in floral and fruit abscission zones during fruit development in tomato (Solanum lycopersicum).
Journal J. Plant Res.
Abstract After fruit development has been triggered by pollination, the abscission zone (AZ) in the pedicel strengthens its adhesion to keep the fruit attached. Unpollinated flowers are shed at their respective AZs, whereas an enlargement of the same tissue is observed in pollinated flowers. After the fruit has developed and is fully ripened, shedding occurs easily at the AZ, indicating an acceleration of abscission. Cell wall degradation and synthesis may play important roles in these processes; however, little is understood. In this report, we have visualized changes in polysaccharide distribution in the AZs of pollinated versus unpollinated flowers and in the ripened fruits using immunohistochemistry. During floral abscission, a large increase was observed in LM15 labeling of xyloglucan specifically at the AZ in the abscising pedicel. LM5 and LM6 labeling of galactan and arabinan, respectively, also increased-LM5 throughout the pedicel and LM6 at the basal side of the AZ. The results suggest that xyloglucan, pectic galactan and arabinan play key roles in the abscission process. During fruit abscission, unlike in floral abscission, no AZ-specific cell wall polysaccharide deposition was observed; however, high autofluorescence was seen in the AZ of over-ripe fruit pedicels, suggesting secondary cell wall synthesis and lignification of the AZ prior to fruit abscission.
Volume 126(3)
Pages 427-37
Published 2013-5
DOI 10.1007/s10265-012-0536-0
PMID 23124772
MeSH Antibodies, Monoclonal / metabolism Cell Wall / metabolism* Flowers / growth & development Flowers / metabolism Fruit / growth & development Fruit / metabolism Glucans / metabolism* Immunohistochemistry Lycopersicon esculentum / growth & development Lycopersicon esculentum / metabolism* Pectins / metabolism* Plant Stems / growth & development Plant Stems / metabolism* Xylans / metabolism*
IF 2.0
Times Cited 8