| RRC ID |
27459
|
| Author |
Liu BB, Tang SK, Zhang YG, Lu XH, Li L, Cheng J, Zhang YM, Zhang LL, Li WJ.
|
| Title |
Halalkalicoccus paucihalophilus sp. nov., a halophilic archaeon from Lop Nur region in Xinjiang, northwest of China.
|
| Journal |
Antonie Van Leeuwenhoek
|
| Abstract |
Two extremely halophilic archaea, designated YIM 93701(T) and YIM 93664, were isolated from Lop Nur region in Xinjiang Province, northwest of China. The cells of the two strains were observed to be cocci, non-motile and Gram-negative. The organisms were determined to be aerobic and required at least 6 % NaCl for growth (optimum 20-25 % and maximum 35 %). Growth was found to occur in the ranges of 16-50 °C (optimum 37 °C) and pH 6.0-8.5 (optimum 6.5-7.5). Cells did not lyse in distilled water. Phylogenetic analysis based on 16S rRNA gene sequences indicated that the two strains belongs to the genus Halalkalicoccus and possessed 99.3 and 99.5 % similarities with their closest phylogenetic relative Halalkalicoccus tibetensis JCM 11890(T). Major polar lipids of the two strains were determined to be phosphatidylglycerol(PG),phosphatidylglycerol phosphate methyl ester (PGP-Me), phosphatidylglycerol sulfate (PGS) and three unidentified glycolipids. The DNA G+C contents were determined to be 60.0-60.4 mol%. The DNA hybridization between the two strains was 92.0 %. In addition, the hybridizations of both strains to H. tibetensis were 49 and 52 %, respectively, and to Halalkalicoccus jeotali were 38 and 33 %, respectively. On the basis of physiological, biochemical tests and phylogenetic differentiations, strains YIM 93701(T) and YIM 93664 were classified as the same species which represent a novel species in the genus Halalkalicoccus, for which the name Halalkalicoccus paucihalophilus sp. nov. is proposed. The type strain is YIM 93701(T) (=JCM 17505(T) = CCTCC 2012803(T)).
|
| Volume |
103(5)
|
| Pages |
1007-14
|
| Published |
2013-5-1
|
| DOI |
10.1007/s10482-013-9880-x
|
| PMID |
23338603
|
| MeSH |
Aerobiosis
Base Composition
China
Cluster Analysis
DNA, Archaeal / chemistry
DNA, Archaeal / genetics
DNA, Ribosomal / chemistry
DNA, Ribosomal / genetics
Halobacteriaceae / classification*
Halobacteriaceae / genetics
Halobacteriaceae / isolation & purification*
Halobacteriaceae / physiology
Hydrogen-Ion Concentration
Molecular Sequence Data
Nucleic Acid Hybridization
Phospholipids / analysis
Phylogeny
RNA, Ribosomal, 16S / genetics
Sequence Analysis, DNA
Sodium Chloride / metabolism
Temperature
|
| IF |
2.674
|
| Times Cited |
13
|
|
WOS Category
|
MICROBIOLOGY
|
| Resource |
| General Microbes |
JCM 11890
JCM 13897
JCM 14584
JCM 15771
JCM 17505 |
