| RRC ID |
27467
|
| 著者 |
Singh NK, Raichand R, Kaur I, Kaur C, Pareek S, Mayilraj S.
|
| タイトル |
Exiguobacterium himgiriensis sp. nov. a novel member of the genus Exiguobacterium, isolated from the Indian Himalayas.
|
| ジャーナル |
Antonie Van Leeuwenhoek
|
| Abstract |
The taxonomic position of an orange coloured bacterium, strain K22-26(T) isolated from a soil sample was studied using a polyphasic approach. The organism had phenotypic and chemotaxonomic properties consistent with its allocation into the genus Exiguobacterium. Phylogenetic analysis of the 16S rRNA gene sequence showed that strain K22-26(T) belongs to the genus Exiguobacterium and was related to Exiguobacterium aurantiacum DSM 6208(T) (99.0 %) Exiguobacterium mexicanum DSM 16483(T) (98.6 %), Exiguobacterium aquaticum (98.6 %), Exiguobacterium aestuarii DSM 16306(T) (98.1 %), Exiguobacterium profundum DSM 17289(T) (98.1 %) and Exiguobacterium marinum DSM 16483(T) (97.9 %), whereas sequence similarity values with respect to other Exiguobacterium species with validly published names were between 92.5-94.0 %. The major polar lipids detected were phosphatidylglycerol, diphosphatidylglycerol and phosphatidylethanolamine. The major menaquinone was determined to be MK-7 (83 %) whereas MK-8 (11 %) and MK-6 (6 %) occur in smaller amounts. The peptidoglycan of the strain was found to contain L-lysine as the diagnostic diamino acid. The major fatty acids detected were iso C13:0 (11.2 %), anteiso C13:0 (15.4 %), iso C15:0 (13.2 %) and iso C17:0 (16.1 %). However, analysis of the DNA-DNA relatedness confirmed that strain K22-26(T) belongs to a novel species. The G + C content of the strain K22-26(T) was determined to be 50.1 mol %. The novel strain was distinguished from closely related type species of the genus Exiguobacterium using DNA-DNA relatedness and phenotypic data. Based on these differences, the strain K22-26(T) should be classified as a novel species of the genus Exiguobacterium, for which the name Exiguobacterium himgiriensis sp. nov. strain K22-26(T) (= MTCC 7628(T) = JCM 14260(T)) is proposed.
|
| 巻・号 |
103(4)
|
| ページ |
789-96
|
| 公開日 |
2013-4-1
|
| DOI |
10.1007/s10482-012-9861-5
|
| PMID |
23229437
|
| MeSH |
Bacillales / classification*
Bacillales / genetics
Bacillales / isolation & purification*
Bacillales / physiology
Bacterial Typing Techniques
Base Composition
Cell Wall / chemistry
Cluster Analysis
Cytosol / chemistry
DNA, Bacterial / chemistry
DNA, Bacterial / genetics
DNA, Ribosomal / chemistry
DNA, Ribosomal / genetics
Fatty Acids / analysis
India
Molecular Sequence Data
Nucleic Acid Hybridization
Phospholipids / analysis
Phylogeny
Pigments, Biological / metabolism
Quinones / analysis
RNA, Ribosomal, 16S / genetics
Sequence Analysis, DNA
Soil Microbiology*
|
| IF |
2.674
|
| 引用数 |
14
|
|
WOS 分野
|
MICROBIOLOGY
|
| リソース情報 |
| 一般微生物 |
JCM 14260 |
