RRC ID 27474
著者 Li Z, Zhao R, Ji S, Shi X, Zhang XH.
タイトル Roseovarius marisflavi sp. nov., isolated from an amphioxus breeding zone in the coastal region of the Yellow Sea, China.
ジャーナル Antonie Van Leeuwenhoek
Abstract A novel Gram stain-negative, catalase- and oxidase-positive, strictly aerobic bacterium, designated strain H50(T), was isolated from an amphioxus breeding zone in the coastal region of the Yellow Sea, China. Cells were observed to be ovoid or short rods, lacked flagella and were found to contain bacteriochlorophyll a. Poly-beta-hydroxybutyrate was found to be accumulated. The temperature range for growth was determined to be 0-37 °C (optimum 28-37 °C). The halotolerance range for growth is 1-15 % NaCl (optimum 2-7 %). The pH range for growth is 6.0-8.0 (optimum 7.0). The major fatty acids were identified as C18:1ω7c and C16:0. The following polar lipids were found to be present: diphosphatidylglycerol, phosphatidylglycerol and a lipid. The predominant respiratory quinone was determined to be Q-10. DNA G+C content was determined to be 57.7 mol%. Strain H50(T) exhibited the highest 16S rRNA gene sequence similarity to Pelagicola litoralis DSM 18290(T) (96.1 %), Roseovarius mucosus DSM 17069(T) (95.8 %) and Roseovarius tolerans DSM 11457(T) (95.7 %). In the phylogenetic trees, strain H50(T) was clustered with the genus Roseovarius but not Pelagicola. On the basis of phenotypic, chemotaxonomic and genotypic data, strain H50(T) is considered to represent a novel species in the genus Roseovarius, for which the name Roseovarius marisflavi sp. nov. is proposed. The type strain is H50(T) (=CGMCC 1.10799(T)=JCM 17553(T)).
巻・号 104(3)
ページ 413-21
公開日 2013-9-1
DOI 10.1007/s10482-013-9965-6
PMID 23828179
MeSH Aerobiosis Animals Bacterial Proteins / analysis Base Composition Catalase / analysis China Cluster Analysis DNA, Bacterial / chemistry DNA, Bacterial / genetics DNA, Ribosomal / chemistry DNA, Ribosomal / genetics Fatty Acids / analysis Hydrogen-Ion Concentration Hydroxybutyrates / analysis Lancelets / growth & development* Molecular Sequence Data Oxidoreductases / analysis Phospholipids / analysis Phylogeny Polyesters / analysis Quinones / analysis RNA, Ribosomal, 16S / genetics Rhodobacteraceae / classification* Rhodobacteraceae / genetics Rhodobacteraceae / isolation & purification* Rhodobacteraceae / physiology Seawater / microbiology* Sequence Analysis, DNA Sodium Chloride / metabolism Temperature
IF 2.674
引用数 18
WOS 分野 MICROBIOLOGY
リソース情報
一般微生物 JCM 17553