RRC ID |
27496
|
著者 |
Suzuki K, Sakamoto H, Shinozaki Y, Tabata J, Watanabe T, Mochizuki A, Koitabashi M, Fujii T, Tsushima S, Kitamoto HK.
|
タイトル |
Affinity purification and characterization of a biodegradable plastic-degrading enzyme from a yeast isolated from the larval midgut of a stag beetle, Aegus laevicollis.
|
ジャーナル |
Appl Microbiol Biotechnol
|
Abstract |
Two yeast strains, which have the ability to degrade biodegradable plastic films, were isolated from the larval midgut of a stag beetle, Aegus laevicollis. Both of them are most closely related to Cryptococcus magnus and could degrade biodegradable plastic (BP) films made of poly(butylene succinate) (PBS) and poly(butylene succinate-co-adipate) (PBSA) effectively. A BP-degrading enzyme was purified from the culture broth of one of the isolated strains employing a newly developed affinity purification method based on the binding action of the enzyme to the substrate (emulsified PBSA) and its subsequent degradative action toward the substrate. Partial amino acid sequences of this enzyme suggested that it belongs to the cutinase family, and thus, the enzyme was named CmCut1. It has a molecular mass of 21 kDa and a degradative activity for emulsified PBSA which was significantly enhanced by the simultaneous presence of Ca(2+) or Mg(2+) at a concentration of about 2.5 mM. Its optimal pH was 7.5, and the optimal temperature was 40 °C. It showed a broad substrate specificity for p-nitrophenyl (pNP)-fatty acid esters ranging from pNP-acetate (C2) to pNP-stearate (C18) and films of PBSA, PBS, poly(ε-caprolactone), and poly(lactic acid).
|
巻・号 |
97(17)
|
ページ |
7679-88
|
公開日 |
2013-9-1
|
DOI |
10.1007/s00253-012-4595-x
|
PMID |
23224497
|
MeSH |
Adipates / metabolism
Amino Acid Sequence
Animals
Biodegradable Plastics / metabolism*
Butylene Glycols / metabolism
Chromatography, Affinity
Coleoptera / growth & development
Coleoptera / microbiology*
Enzyme Stability
Fungal Proteins / chemistry*
Fungal Proteins / genetics
Fungal Proteins / metabolism*
Gastrointestinal Tract / microbiology
Larva / microbiology
Molecular Sequence Data
Molecular Weight
Polymers / metabolism
Sequence Alignment
Substrate Specificity
Succinates / metabolism
Yeasts / chemistry
Yeasts / enzymology*
Yeasts / isolation & purification*
Yeasts / metabolism
|
IF |
3.53
|
引用数 |
17
|
WOS 分野
|
BIOTECHNOLOGY & APPLIED MICROBIOLOGY
|
リソース情報 |
一般微生物 |
JCM 9038
JCM 10317
JCM 10631 |