RRC ID |
27503
|
Author |
Chen YS, Yu CR, Ji SH, Liou MS, Leong KH, Pan SF, Wu HC, Lin YH, Yu B, Yanagida F.
|
Title |
Enterocin T, a novel class IIa bacteriocin produced by Enterococcus sp. 812.
|
Journal |
Arch Microbiol
|
Abstract |
Enterococcus sp. 812, isolated from fresh broccoli, was previously found to produce a bacteriocin active against a number of Gram-positive bacteria, including Listeria monocytogenes. Bacteriocin activity decreased slightly after autoclaving (121 °C for 15 min), but was inactivated by protease K. Mass spectrometry analysis revealed the bacteriocin mass to be approximately 4,521.34 Da. N-terminal amino acid sequencing yielded a partial sequence, NH2-ATYYGNGVYXDKKKXWVEWGQA, by Edman degradation, which contained the consensus class IIa bacteriocin motif YGNGV in the N-terminal region. The obtained partial sequence showed high homology with some enterococcal bacteriocins; however, no identical peptide or protein was found. This peptide was therefore considered to be a novel bacteriocin produced by Enterococcus sp. 812 and was termed enterocin T.
|
Volume |
195(9)
|
Pages |
655-60
|
Published |
2013-9-1
|
DOI |
10.1007/s00203-013-0917-3
|
PMID |
23912616
|
MeSH |
Amino Acid Motifs
Amino Acid Sequence
Bacteriocins / chemistry
Bacteriocins / isolation & purification*
Bacteriocins / metabolism*
Enterococcus / chemistry
Enterococcus / metabolism
Gram-Positive Bacteria
Sequence Alignment
|
IF |
1.884
|
Times Cited |
3
|
WOS Category
|
MICROBIOLOGY
|
Resource |
General Microbes |
JCM 1157 |