Reference - Detail
|Author||Toshimitsu T, Nakamura M, Ikegami S, Terahara M, Itou H.|
|Title||Strain-specific identification of Bifidobacterium bifidum OLB6378 by PCR.|
|Journal||Biosci Biotechnol Biochem|
The aim of the present study was to develop a strain-specific polymerase chain reaction (PCR) primer set for the detection of Bifidobacterium bifidum OLB6378 (OLB6378) that can serve as suitable probiotics for infants. The random amplified polymorphic DNA (RAPD)-PCR technique was used to obtain OLB6378-specific PCR products. One OLB6378-specific RAPD-PCR product was obtained after testing 97 RAPD primers, and was sequenced. Thirteen PCR primer sets were designed from the sequence. One PCR primer set was found to amplify one PCR product when genomic DNA of OLB6378 was used as template. The primer set did not amplify any PCR product when the other genomic DNA was used as template. The primer set was tested with 47 strains of B. bifidum and 20 strains of the other Bifidobacterium species. As a result, we developed an OLB6378-specific primer set, one that should be useful not only for the detection of OLB6378 but also for the quantification of OLB6378.
|MeSH||Bifidobacterium / genetics* Bifidobacterium / isolation & purification* DNA Primers / genetics* Feces / microbiology Humans Infant Limit of Detection Probiotics / isolation & purification Random Amplified Polymorphic DNA Technique Real-Time Polymerase Chain Reaction / methods* Species Specificity|
|WOS Category||FOOD SCIENCE & TECHNOLOGY BIOTECHNOLOGY & APPLIED MICROBIOLOGY CHEMISTRY, APPLIED BIOCHEMISTRY & MOLECULAR BIOLOGY|
|General Microbes||JCM 1190 JCM 1192 JCM 1194 JCM 1195 JCM 1209 JCM 1217 JCM 1222 JCM 1254 JCM 1255 JCM 1272 JCM 1275 JCM 7002 JCM 7003 JCM 7004 JCM 7054 JCM 7096 JCM 7135 JCM 10602|