論文 - 詳細
| RRC ID | 27518 |
|---|---|
| 著者 | Hashimoto M, Eguchi H, Tawaratsumida K, Kirikae T, Suda Y. |
| タイトル | Identification of a TLR2-stimulating lipoprotein in Bacteroides fragilis JCM 11019 (NCTC 9343). |
| ジャーナル | Innate Immun |
| Abstract |
Bacteroides fragilis is found among the normal intestinal flora and is involved in host immunostimulation via TLR2. Its cell surface components, such as LPS and capsular polysaccharides, were reported to participate in host immunostimulation. In this study, we report on the existence of a lipoprotein that acts as a TLR2 stimulant in B. fragilis. The TLR2-stimulating lipoprotein was obtained using Triton X-114-water phase partitioning followed by preparative SDS-PAGE. Its N-terminal hydrophobic peptide, which was separated from a tryptic digest, was characterized as a triacylated lipopeptide, and the lipoprotein was identified as BF1333 by mass spectrometry of Asp-N-digested peptides. These results showed that the lipoprotein acts as a TLR2-stimulating component in B. fragilis. |
| 巻・号 | 19(2) |
| ページ | 132-9 |
| 公開日 | 2013-1-1 |
| DOI | 10.1177/1753425912454179 |
| PII | 1753425912454179 |
| PMID | 22890547 |
| MeSH | Acylation Animals Antigens, Bacterial / chemistry Antigens, Bacterial / immunology* Antigens, Bacterial / isolation & purification Bacteroides fragilis / immunology* Electrophoresis, Polyacrylamide Gel Immunization Lipoproteins / chemistry Lipoproteins / immunology* Lipoproteins / isolation & purification Macrophages, Peritoneal / immunology* Male Mass Spectrometry Mice Mice, Inbred BALB C Octoxynol Peptide Fragments / chemistry Peptide Fragments / immunology* Peptide Fragments / isolation & purification Polyethylene Glycols / chemistry Toll-Like Receptor 2 / immunology Toll-Like Receptor 2 / metabolism* |
| IF | 2.298 |
| 引用数 | 3 |
| WOS 分野 | MEDICINE, RESEARCH & EXPERIMENTAL MICROBIOLOGY IMMUNOLOGY BIOCHEMISTRY & MOLECULAR BIOLOGY |
| リソース情報 | |
| 一般微生物 | JCM 11019 |