RRC ID 2767
Author Ihara H, Kuwamura M, Atsuta M, Nihonmatsu I, Okada T, Mukamoto M, Kozaki S.
Title Expression of neuronal nitric oxide synthase variant, nNOS-mu, in rat brain.
Journal Nitric Oxide
Abstract Neuronal nitric oxide synthase (nNOS) is alternatively spliced. An nNOS splice variant form, nNOS-mu, was first found to be selectively expressed in rat skeletal muscle and heart. To date, the expression of nNOS-mu in the brain has not been well characterized. The aim of this study was to determine whether nNOS-mu is expressed in rat brain, and whether nNOS-mu exhibits a specific expression pattern. To analyze the expression of nNOS-mu, we generated a monoclonal antibody that is specific for nNOS-mu. An immunoblot analysis using this antibody showed that nNOS-mu is expressed in the rat brain at a measurable level, which was 10.3% of total nNOSs. In rat brain, the nNOS-mu expression was high in the mesencephalon and the cerebellum. nNOS-mu was immunohistochemically localized in neurites and perikarya of large neurons. In the cerebellum, granule cells showed marked staining, while weak staining was detected in basket and stellate cells. This expression pattern is different from that described for nNOS and suggests that nNOS-mu plays unique roles in different neurons.
Volume 15(1)
Pages 13-9
Published 2006-8
DOI 10.1016/j.niox.2005.11.011
PII S1089-8603(05)00176-X
PMID 16412669
MeSH Alternative Splicing Animals Antibodies, Monoclonal Brain / cytology Brain / enzymology* Cerebellum / cytology Cerebellum / enzymology Immunoblotting Immunohistochemistry Isoenzymes / analysis Mice Nitric Oxide Synthase Type I / analysis* Nitric Oxide Synthase Type I / genetics Nitric Oxide Synthase Type I / immunology Rats
IF 3.371
Times Cited 9