RRC ID 27702
Author Khunthongpan S, Bourneow C, H-Kittikun A, Tanasupawat S, Benjakul S, Sumpavapol P.
Title Enterobacter siamensis sp. nov., a transglutaminase-producing bacterium isolated from seafood processing wastewater in Thailand.
Journal J Gen Appl Microbiol
Abstract A novel strain of Enterobacter, C2361(T), a Gram-negative, non-spore-forming, rod-shaped and facultative anaerobic bacterium with the capability to produce transglutaminase, was isolated from seafood processing wastewater collected from a treatment pond of a seafood factory in Songkhla Province, Thailand. Phylogenetic analyses and phenotypic characteristics, including chemotaxonomic characteristics, showed that the strain was a member of the genus Enterobacter. The 16S rRNA gene sequence similarities between strain C2361(T) and Enterobacter cloacae subsp. cloacae ATCC 13047(T) and Enterobacter cloacae subsp. dissolvens LMG 2683(T) were 97.5 and 97.5%, respectively. Strain C2361(T) showed a low DNA-DNA relatedness with the above-mentioned species. The major fatty acids were C16:0, C17:0cyclo and C14:0. The DNA G+C content was 53.0 mol%. On the basis of the polyphasic evidence gathered in this study, it should be classified as a novel species of the genus Enterobacter for which the name Enterobacter siamensis sp. nov. is proposed. The type strain is C2361(T) (= KCTC 23282(T) = NBRC 107138(T)).
Volume 59(2)
Pages 135-40
Published 2013-1-1
DOI 10.2323/jgam.59.135
PII DN/JST.JSTAGE/jgam/59.135
PMID 23759866
MeSH Aerobiosis Anaerobiosis Bacterial Typing Techniques Base Composition Cluster Analysis DNA, Bacterial / chemistry DNA, Bacterial / genetics DNA, Ribosomal / chemistry DNA, Ribosomal / genetics Enterobacter / classification* Enterobacter / enzymology* Enterobacter / genetics Enterobacter / isolation & purification Fatty Acids / analysis Industrial Waste Microscopy, Electron, Scanning Microscopy, Phase-Contrast Molecular Sequence Data Nucleic Acid Hybridization Phylogeny RNA, Ribosomal, 16S / genetics Seafood Sequence Analysis, DNA Thailand Transglutaminases / metabolism* Waste Water / microbiology*
IF 1.442
Times Cited 5
WOS Category BIOTECHNOLOGY & APPLIED MICROBIOLOGY MICROBIOLOGY
Resource
General Microbes JCM 6049