RRC ID 27731
Author Ohmori I, Hayashi K, Wang H, Ouchida M, Fujita N, Inoue T, Michiue H, Nishiki T, Matsui H.
Title Inhalation of 10% carbon dioxide rapidly terminates Scn1a mutation-related hyperthermia-induced seizures.
Journal Epilepsy Res.
Abstract The aim of this study was to assess the anticonvulsant effect of carbon dioxide (CO2) on Scn1a mutation-related febrile seizures. We examined physiological changes in the blood gas levels after the induction of hyperthermia-induced seizures (HISs), which were associated with the Scn1a missense mutation. We determined the efficacy of inhalation of 5% or 10% CO2 to treat HISs. HISs were evoked in Scn1a mutant and wild-type (WT) rats by hot water baths. To determine the anticonvulsant effect of CO2 inhalation, rats were placed in a chamber filled with air or mixed gas containing 5% CO2 or 10% CO2 for 3 min, immediately after the induction of HISs. We also analyzed the blood gas levels at the end of inhalation of CO2. Hot water bathing induced a significant reduction in the partial pressure of CO2 (pCO2) and respiratory alkalosis in the WT and Scn1a mutant rats. HISs were evoked in 100% of the Scn1a mutant rats within 5 min, but in none of the WT rats. The Scn1a mutant rats demonstrated a higher HISs susceptibility associated with respiratory alkalosis than the WT rats. Inhalation of 10% CO2 shortened the seizure duration from 62.6±12.1 s to 15.5±1.0 s. Blood gas analysis after the inhalation of 10% CO2 demonstrated an elevated pCO2 level and respiratory acidosis. Inhalation of 10% CO2 demonstrated a potent and fast-acting anticonvulsant effect against HISs.
Volume 105(1-2)
Pages 220-4
Published 2013-7
DOI 10.1016/j.eplepsyres.2013.01.003
PII S0920-1211(13)00016-8
PMID 23375560
MeSH Animals Carbon Dioxide / administration & dosage* Fever / drug therapy Fever / genetics* Fever / metabolism Inhalation / physiology Male Mutation, Missense / genetics* NAV1.1 Voltage-Gated Sodium Channel / genetics* Rats Rats, Inbred F344 Rats, Mutant Strains Seizures / drug therapy Seizures / genetics* Seizures / metabolism Time Factors
IF 2.178
Times Cited 5
Rats F344-Scn1am1Kyo(strainID=716)