RRC ID |
27731
|
Author |
Ohmori I, Hayashi K, Wang H, Ouchida M, Fujita N, Inoue T, Michiue H, Nishiki T, Matsui H.
|
Title |
Inhalation of 10% carbon dioxide rapidly terminates Scn1a mutation-related hyperthermia-induced seizures.
|
Journal |
Epilepsy Res
|
Abstract |
The aim of this study was to assess the anticonvulsant effect of carbon dioxide (CO2) on Scn1a mutation-related febrile seizures. We examined physiological changes in the blood gas levels after the induction of hyperthermia-induced seizures (HISs), which were associated with the Scn1a missense mutation. We determined the efficacy of inhalation of 5% or 10% CO2 to treat HISs. HISs were evoked in Scn1a mutant and wild-type (WT) rats by hot water baths. To determine the anticonvulsant effect of CO2 inhalation, rats were placed in a chamber filled with air or mixed gas containing 5% CO2 or 10% CO2 for 3 min, immediately after the induction of HISs. We also analyzed the blood gas levels at the end of inhalation of CO2. Hot water bathing induced a significant reduction in the partial pressure of CO2 (pCO2) and respiratory alkalosis in the WT and Scn1a mutant rats. HISs were evoked in 100% of the Scn1a mutant rats within 5 min, but in none of the WT rats. The Scn1a mutant rats demonstrated a higher HISs susceptibility associated with respiratory alkalosis than the WT rats. Inhalation of 10% CO2 shortened the seizure duration from 62.6±12.1 s to 15.5±1.0 s. Blood gas analysis after the inhalation of 10% CO2 demonstrated an elevated pCO2 level and respiratory acidosis. Inhalation of 10% CO2 demonstrated a potent and fast-acting anticonvulsant effect against HISs.
|
Volume |
105(1-2)
|
Pages |
220-4
|
Published |
2013-7-1
|
DOI |
10.1016/j.eplepsyres.2013.01.003
|
PII |
S0920-1211(13)00016-8
|
PMID |
23375560
|
MeSH |
Animals
Carbon Dioxide / administration & dosage*
Fever / drug therapy
Fever / genetics*
Fever / metabolism
Inhalation / physiology
Male
Mutation, Missense / genetics*
NAV1.1 Voltage-Gated Sodium Channel / genetics*
Rats
Rats, Inbred F344
Rats, Mutant Strains
Seizures / drug therapy
Seizures / genetics*
Seizures / metabolism
Time Factors
|
IF |
2.208
|
Times Cited |
5
|
WOS Category
|
CLINICAL NEUROLOGY
|
Resource |
Rats |
F344-Scn1am1Kyo(strainID=716) |