Reference - Detail
| RRC ID | 27740 |
|---|---|
| Author | Mashimo T, Takizawa A, Kobayashi J, Kunihiro Y, Yoshimi K, Ishida S, Tanabe K, Yanagi A, Tachibana A, Hirose J, Yomoda J, Morimoto S, Kuramoto T, Voigt B, Watanabe T, Hiai H, Tateno C, Komatsu K, Serikawa T. |
| Title | Generation and characterization of severe combined immunodeficiency rats. |
| Journal | Cell Rep |
| Abstract |
Severe combined immunodeficiency (SCID) mice, the most widely used animal model of DNA-PKcs (Prkdc) deficiency, have contributed enormously to our understanding of immunodeficiency, lymphocyte development, and DNA-repair mechanisms, and they are ideal hosts for allogeneic and xenogeneic tissue transplantation. Here, we use zinc-finger nucleases to generate rats that lack either the Prkdc gene (SCID) or the Prkdc and Il2rg genes (referred to as F344-scid gamma [FSG] rats). SCID rats show several phenotypic differences from SCID mice, including growth retardation, premature senescence, and a more severe immunodeficiency without "leaky" phenotypes. Double-knockout FSG rats show an even more immunocompromised phenotype, such as the abolishment of natural killer cells. Finally, xenotransplantation of human induced pluripotent stem cells, ovarian cancer cells, and hepatocytes shows that SCID and FSG rats can act as hosts for xenogeneic tissue grafts and stem cell transplantation and may be useful for preclinical testing of new drugs. |
| Volume | 2(3) |
| Pages | 685-94 |
| Published | 2012-9-27 |
| DOI | 10.1016/j.celrep.2012.08.009 |
| PII | S2211-1247(12)00234-3 |
| PMID | 22981234 |
| MeSH | Animals DNA-Activated Protein Kinase / genetics DNA-Activated Protein Kinase / metabolism Disease Models, Animal* Gene Knockout Techniques Humans Interleukin Receptor Common gamma Subunit / genetics Interleukin Receptor Common gamma Subunit / metabolism Mice Mice, SCID Pluripotent Stem Cells / metabolism Pluripotent Stem Cells / transplantation Rats Rats, Mutant Strains Severe Combined Immunodeficiency* Transplantation, Heterologous |
| IF | 8.109 |
| Times Cited | 37 |
| WOS Category | CELL BIOLOGY |
| Altmetric score |
オルトメトリクス指標項目
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| The most frequently cited source | Patent(IFI CLAIMS) |
| Total number of mentions | 14 |
| Altmetric score changes over past 6months | 0.0 |
| Resource | |
| Rats | F344-Il2rgem2Kyo(strainID=979) |