| RRC ID |
27741
|
| Author |
Kodama A, Kamei N, Kamei G, Kongcharoensombat W, Ohkawa S, Nakabayashi A, Ochi M.
|
| Title |
In vivo bioluminescence imaging of transplanted bone marrow mesenchymal stromal cells using a magnetic delivery system in a rat fracture model.
|
| Journal |
J Bone Joint Surg Br
|
| Abstract |
For the treatment of ununited fractures, we developed a system of delivering magnetic labelled mesenchymal stromal cells (MSCs) using an extracorporeal magnetic device. In this study, we transplanted ferucarbotran-labelled and luciferase-positive bone marrow-derived MSCs into a non-healing femoral fracture rat model in the presence of a magnetic field. The biological fate of the transplanted MSCs was observed using luciferase-based bioluminescence imaging and we found that the number of MSC derived photons increased from day one to day three and thereafter decreased over time. The magnetic cell delivery system induced the accumulation of photons at the fracture site, while also retaining higher photon intensity from day three to week four. Furthermore, radiological and histological findings suggested improved callus formation and endochondral ossification. We therefore believe that this delivery system may be a promising option for bone regeneration.
|
| Volume |
94(7)
|
| Pages |
998-1006
|
| Published |
2012-7-1
|
| DOI |
10.1302/0301-620X.94B7.28521
|
| PII |
94-B/7/998
|
| PMID |
22733960
|
| MeSH |
Animals
Bone Regeneration / physiology
Cell Differentiation / physiology
Cell Movement
Dextrans
Disease Models, Animal
Female
Femoral Fractures / pathology
Femoral Fractures / physiopathology
Femoral Fractures / therapy*
Fracture Healing / physiology*
Fractures, Ununited / pathology
Fractures, Ununited / physiopathology
Fractures, Ununited / therapy*
Luminescent Measurements / methods
Magnetic Fields*
Magnetite Nanoparticles
Mesenchymal Stem Cell Transplantation*
Mesenchymal Stem Cells / pathology
Mesenchymal Stem Cells / physiology
Rats
Rats, Inbred Lew
|
| Times Cited |
27
|
|
WOS Category
|
SURGERY
ORTHOPEDICS
|
| Resource |
| Rats |
LEW-Tg(Gt(ROSA)26Sor-luc)11Jmsk(strainID=649)
LEW-Tg(Gt(ROSA)26Sor-lacZ)44Jmsk(strainID=648) |
