論文 - 詳細
| RRC ID | 2783 |
|---|---|
| 著者 | Nakasone N, Yoshie H, Ohshima H. |
| タイトル | An immunohistochemical study of the expression of heat-shock protein-25 and cell proliferation in the dental pulp and enamel organ during odontogenesis in rat molars. |
| ジャーナル | Arch Oral Biol |
| Abstract |
OBJECTIVES:The aim of this study is to clarify the functional significance of heat-shock protein (HSP)-25 during tooth development. DESIGN:We compared the expression of HSP-25 in the dental epithelial and mesenchymal cells with their proliferative activity during odontogenesis in rat molars on postnatal days 1-100 by immunohistochemistry using anti-HSP-25 and anti-5-bromo-2'-deoxyuridine (BrdU) for cell proliferation assay. RESULTS:On day 1, BrdU-immunoreactive cells were densely located in the inner enamel epithelium in the cervical loop and intercusped areas and the dental pulp adjacent to them, whereas HSP-25-immunoractivity (IR) was restricted to the cusped area where odontoblasts and ameloblasts had already differentiated. Subsequently, BrdU-IR shifted in the apical direction to be localized around Hertwig's epithelial root sheath during days 5-30, never overlapping with concomitantly apically-shifted HSP-25-IR. On days 60-100, BrdU-immunoreactive cells were hardly recognizable in the dental pulp, where HSP-25-IR was exclusively localized in the odontoblast layer. Furthermore, the odontoblast- and ameloblast-lineage cells exhibited two steps in the expression of HSP-25 throughout the postnatal stages: first, dental epithelial and pulpal mesenchymal cells showed a weak IR for HSP-25 after the cessation of their proliferative activity, and subsequently odontoblasts and ameloblasts consistently expressed an intense HSP-25-IR. CONCLUSION:Odontoblast- and ameloblast-lineage cells acquire HSP-25-IR after they complete their cell division, suggesting that this protein acts as a switch between cell proliferation and differentiation during tooth development. The consistent expression of HSP-25-IR in the formative cells may be involved in the maintenance of their functional integrity. |
| 巻・号 | 51(5) |
| ページ | 378-86 |
| 公開日 | 2006-5-1 |
| DOI | 10.1016/j.archoralbio.2005.09.007 |
| PII | S0003-9969(05)00239-6 |
| PMID | 16259940 |
| MeSH | Amelogenesis / physiology Animals Animals, Newborn Bromodeoxyuridine / analysis Cell Proliferation Dental Pulp / chemistry Dental Pulp / growth & development* Dentinogenesis / physiology Enamel Organ / chemistry Enamel Organ / growth & development* HSP27 Heat-Shock Proteins Heat-Shock Proteins / analysis* Immunohistochemistry / methods Molar / chemistry Molar / growth & development* Neoplasm Proteins / analysis* Odontogenesis / physiology* Rats Rats, Sprague-Dawley |
| IF | 1.931 |
| 引用数 | 31 |
| WOS 分野 | DENTISTRY, ORAL SURGERY & MEDICINE |
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