Reference - Detail
| RRC ID | 27848 |
|---|---|
| Author | Donia MS, Schmidt EW. |
| Title | Linking chemistry and genetics in the growing cyanobactin natural products family. |
| Journal | Chem Biol |
| Abstract |
Ribosomal peptide natural products are ubiquitous, yet relatively few tools exist to predict structures and clone new pathways. Cyanobactin ribosomal peptides are found in ~30% of all cyanobacteria, but the connection between gene sequence and structure was not defined, limiting the rapid identification of new compounds and pathways. Here, we report discovery of four orphan cyanobactin gene clusters by genome mining and an additional pathway by targeted cloning, which represented a tyrosine O-prenylating biosynthetic pathway. Genome mining enabled discovery of five cyanobactins, including peptide natural products from Spirulina supplements. A phylogenetic model defined four cyanobactin genotypes, which explain the synthesis of multiple cyanobactin structural classes and help direct pathway cloning and structure prediction efforts. These strategies were applied to DNA isolated from a mixed cyanobacterial bloom containing cyanobactins. |
| Volume | 18(4) |
| Pages | 508-19 |
| Published | 2011-4-22 |
| DOI | 10.1016/j.chembiol.2011.01.019 |
| PII | S1074-5521(11)00085-8 |
| PMID | 21513887 |
| PMC | PMC3119926 |
| MeSH | Amino Acid Sequence Biological Products / biosynthesis Biological Products / chemistry* Biological Products / genetics* Cyanobacteria / cytology Cyanobacteria / genetics* Cyanobacteria / metabolism Dietary Supplements / microbiology Genome, Bacterial / genetics Metagenomics Models, Biological Molecular Sequence Data Multigene Family / genetics Peptides / chemistry* Peptides / genetics* Peptides / metabolism Phylogeny Ribosomal Proteins / biosynthesis Ribosomal Proteins / chemistry* Ribosomal Proteins / genetics* Sequence Analysis, DNA |
| IF | 5.915 |
| Times Cited | 65 |
| WOS Category | BIOCHEMISTRY & MOLECULAR BIOLOGY |
| Resource | |
| Algae | NIES-596 |