RRC ID 27854
著者 Koskenniemi K, Lyra C, Rajaniemi-Wacklin P, Jokela J, Sivonen K.
タイトル Quantitative real-time PCR detection of toxic Nodularia cyanobacteria in the Baltic Sea.
ジャーナル Appl Environ Microbiol
Abstract A specific quantitative real-time PCR (qPCR) method was developed for the quantification of hepatotoxin nodularin-producing Nodularia, one of the main bloom-forming cyanobacteria in the Baltic Sea. Specific PCR primers were designed for subunit F of the nodularin synthetase gene (ndaF), which encodes the NdaF subunit of the nodularin synthetase gene complex needed for nodularin production. The qPCR method was applied to water samples (a total of 120 samples) collected from the Baltic Sea in July 2004. As few as 30 ndaF gene copies ml(-1) of seawater could be detected, and thus, the method was very sensitive. The ndaF gene copy numbers and nodularin concentrations were shown to correlate in the Baltic seawater, indicating the constant production of nodularin by Nodularia. This qPCR method for the ndaF gene can be used for detailed studies of Nodularia blooms and their formation. ndaF gene copies and nodularin were detected mostly in the surface water but also in deeper water layers (down to 30 m). Toxic Nodularia blooms are not only horizontally but also vertically widely distributed, and thus, the Baltic fauna is extensively exposed to nodularin.
巻・号 73(7)
ページ 2173-9
公開日 2007-4-1
DOI 10.1128/AEM.02746-06
PII AEM.02746-06
PMID 17277219
PMC PMC1855639
MeSH DNA Primers DNA, Bacterial / analysis Nodularia / genetics Nodularia / growth & development Nodularia / isolation & purification* Peptides, Cyclic / biosynthesis Polymerase Chain Reaction / methods* Seawater / microbiology*
IF 4.016
引用数 64
WOS 分野 BIOTECHNOLOGY & APPLIED MICROBIOLOGY MICROBIOLOGY
リソース情報
藻類 NIES-81 NIES-102 NIES-89