RRC ID 27859
Author Suda T, Hata T, Kawai S, Okamura H, Nishida T.
Title Treatment of tetracycline antibiotics by laccase in the presence of 1-hydroxybenzotriazole.
Journal Bioresour Technol
Abstract Tetracycline antibiotics are widely used in human and veterinary medicine; however, residual amounts of these antibiotics in the environment are of concern since they could contribute to selection of resistant bacteria. In this study, tetracycline (TC), chlortetracycline (CTC), doxycycline (DC) and oxytetracycline (OTC) were treated with laccase from the white rot fungus Trametes versicolor in the presence of the redox mediator 1-hydroxybenzotriazole (HBT). High performance liquid chromatography demonstrated that DC and CTC were completely eliminated after 15 min, while TC and CTC were eliminated after 1 h. This system also resulted in a complete loss of inhibition of growth of Escherichia coli and Bacillus subtilis and the green alga Pseudokirchneriella subcapitata with decreasing tetracycline antibiotic concentration. These results suggest that the laccase-HBT system is effective in eliminating tetracycline antibiotics and removing their ecotoxicity.
Volume 103(1)
Pages 498-501
Published 2012-1-1
DOI 10.1016/j.biortech.2011.10.041
PII S0960-8524(11)01507-0
PMID 22071243
MeSH Anti-Bacterial Agents / pharmacology* Bacillus subtilis / drug effects Bacillus subtilis / growth & development Bacillus subtilis / metabolism Chlorophyta / drug effects Chlorophyta / growth & development Escherichia coli / drug effects Escherichia coli / growth & development Escherichia coli / metabolism Humans Laccase / metabolism* Lignin / metabolism Tetracycline / pharmacology* Triazoles / pharmacology*
IF 7.539
Times Cited 66
Algae NIES-35