論文 - 詳細
| RRC ID | 27864 |
|---|---|
| 著者 | Yamamoto Y, Kawashima D, Hashizume A, Hisamatsu M, Isono N. |
| タイトル | Purification and characterization of 1,3-β-D-glucan phosphorylase from Ochromonas danica. |
| ジャーナル | Biosci Biotechnol Biochem |
| Abstract |
1,3-β-D-glucan phosphorylase (BGP) is an enzyme that catalyzes the reversible phosphorolysis of 1,3-β-glucosidic linkages to form α-D-glucose 1-phosphate (G1P). Here we report on the purification and characterization of BGP from Ochromonas danica (OdBGP). The purified enzyme preparation showed three bands (113, 118, and 124 kDa) on SDS-polyacrylamide gel electrophoresis. The optimum pH and temperature were 5.5 and 25 °C-30 °C. OdBGP phosphorolysed laminaritriose, larger laminarioligosaccharides, and laminarin, but not laminaribiose. In the synthesis reaction, laminarin and laminarioligosaccharides served as good acceptors, but OdBGP did not act on glucose. Kinetic analysis indicated that the phosphorolysis reaction of OdBGP follows a sequential Bi Bi mechanism. The equilibrium of the enzymatic reaction indicated that OdBGP favors the reaction in the synthetic direction. Overnight incubation of OdBGP with laminaribiose and G1P resulted in the formation of precipitates, which were probably 1,3-β-glucans. |
| 巻・号 | 77(9) |
| ページ | 1949-54 |
| 公開日 | 2013-1-1 |
| DOI | 10.1271/bbb.130411 |
| PII | DN/JST.JSTAGE/bbb/130411 |
| PMID | 24018693 |
| MeSH | Disaccharides / metabolism Glucosephosphates / metabolism Hydrogen-Ion Concentration Kinetics Ochromonas / enzymology* Phosphorylases / isolation & purification* Phosphorylases / metabolism* Proteoglycans Substrate Specificity Temperature beta-Glucans / metabolism |
| IF | 1.516 |
| 引用数 | 7 |
| WOS 分野 | FOOD SCIENCE & TECHNOLOGY BIOTECHNOLOGY & APPLIED MICROBIOLOGY CHEMISTRY, APPLIED BIOCHEMISTRY & MOLECULAR BIOLOGY |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 最多言及媒体 | Patent(IFI CLAIMS) |
| 各媒体での言及数の合計 | 4 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| 藻類 | NIES-2142 |