RRC ID 27869
Author Tian Y, Luo C, Ouyang Q.
Title A microfluidic synchronizer for fission yeast cells.
Journal Lab Chip
Abstract Among all the cell cycle synchronization technologies, the baby machine may be considered as the most artifact-free method. A baby machine incubates "mother cells" under normal conditions and collects their "babies", producing cell cultures that are similar not only in cell cycle phase but also in age. Unlike many other synchronization methods, no cell-cycle-blocking agent or metabolic stress is introduced in this method. Several macroscale and microfluidic baby machines have been developed for producing synchronized cell colonies. However, for rod-shaped cells like fission yeast (Schizosaccharomyces pombe), it is still a challenge to immobilize only the mother cells in a microfluidic device. Here we presented a new baby machine suitable for fission yeast. The device is fixed one end of the cell and releases the free-end daughter cell every time the cell finishes cytokinesis. A variety of structures for cell immobilization were attempted to find the optimal design. For the convenience of collection and further assay, we integrated into our baby machine chip a cell screener, which exploited the deformation of polymer material to switch between opening and closing states. Synchronous populations of fission yeast cells were produced with this device, its working detail was analyzed and performance was evaluated. The device provides a new on-chip tool for cell biology studies.
Volume 13(20)
Pages 4071-7
Published 2013-10-21
DOI 10.1039/c3lc50639h
PMID 23966136
MeSH Centrifugation Cytological Techniques / methods* Pressure Schizosaccharomyces / cytology* Tissue Array Analysis / methods*
IF 6.914
Times Cited 12
WOS Category BIOCHEMICAL RESEARCH METHODS CHEMISTRY, MULTIDISCIPLINARY NANOSCIENCE & NANOTECHNOLOGY
Resource
Yeast NA