Reference - Detail
| RRC ID | 27883 |
|---|---|
| Author | Tian F, Morimoto N, Liu W, Ohta Y, Deguchi K, Miyazaki K, Abe K. |
| Title | In vivo optical imaging of motor neuron autophagy in a mouse model of amyotrophic lateral sclerosis. |
| Journal | Autophagy |
| Abstract |
Autophagy is involved in the pathological process of motor neuron death in amyotrophic lateral sclerosis (ALS). We have generated a novel double transgenic (DTg) mouse line by mating a green fluorescent protein (GFP)-fused microtubule-associated protein 1 light chain 3 (LC3) transgenic (LC3-Tg) mouse and a G93A mutant human Cu/Zn superoxide dismutase (mSOD1) transgenic (mSOD1-Tg) mouse. In vivo imaging of autophagy with these novel DTg mice was conducted at 10 (presymptomatic), 17 (early symptomatic) and 19 (late symptomatic) weeks of age. Fluorescence imaging analysis revealed a strong fluorescent signal in vivo over the T₃-S₁ level at 17 and 19 weeks of age only in the DTg mice. Ex vivo autophagy imaging of spinal cord sections (20 μm) also showed a progressive increase of the fluorescence signal from 17 to 19 weeks in DTg mice in the anterior horn at the L₄-₅ level, and the fluorescence signals were clearly observed in the gray matter of the spinal cord with a progressive increase of the signal and decreases in large motor neurons. Protein gel blot analysis revealed maximum LC3-I and LC3-II expressions at 19 weeks, consistent with the results from the in vivo autophagy imaging experiment. This method could also be applied as a unique tool for clarifying the role of autophagy, and to monitor the pathologic processes involving autophagy not only in ALS, but also other neurological diseases. |
| Volume | 7(9) |
| Pages | 985-92 |
| Published | 2011-9-1 |
| DOI | 10.4161/auto.7.9.16012 |
| PII | 16012 |
| PMID | 21628996 |
| MeSH | Amyotrophic Lateral Sclerosis / diagnosis* Amyotrophic Lateral Sclerosis / pathology* Animals Astrocytes / metabolism Astrocytes / pathology Autophagy* Cell Count Diagnostic Imaging / methods* Disease Models, Animal Fluorescent Antibody Technique Green Fluorescent Proteins / metabolism Humans Mice Mice, Transgenic Microtubule-Associated Proteins / metabolism Motor Neurons / pathology* Optical Phenomena* Phagosomes / metabolism Recombinant Fusion Proteins / metabolism |
| IF | 9.77 |
| Times Cited | 37 |
| WOS Category | CELL BIOLOGY |
| Altmetric score |
オルトメトリクス指標項目
|
| The most frequently cited source | Patent(IFI CLAIMS) |
| Total number of mentions | 1 |
| Altmetric score changes over past 6months | 0.0 |
| Resource | |
| Mice | RBRC00806 |