RRC ID 27902
Author Hirayama M, Ogawa M, Oshima M, Sekine Y, Ishida K, Yamashita K, Ikeda K, Shimmura S, Kawakita T, Tsubota K, Tsuji T.
Title Functional lacrimal gland regeneration by transplantation of a bioengineered organ germ.
Journal Nat Commun
Abstract The lacrimal gland has a multifaceted role in maintaining a homeostatic microenvironment for a healthy ocular surface via tear secretion. Dry-eye disease, which is caused by lacrimal gland dysfunction, is one of the most prevalent eye diseases that cause corneal epithelial damage and results in significant loss of vision and a reduction in the quality of life. Here we demonstrate orthotopic transplantation of bioengineered lacrimal gland germs into adult mice with an extra-orbital lacrimal gland defect, a mouse model that mimics the corneal epithelial damage caused by lacrimal gland dysfunction. The bioengineered lacrimal gland germs and harderian gland germs both develop in vivo and achieve sufficient physiological functionality, including tear production in response to nervous stimulation and ocular surface protection. This study demonstrates the potential for bioengineered organ replacement to functionally restore the lacrimal gland.
Volume 4
Pages 2497
Published 2013-1-1
DOI 10.1038/ncomms3497
PII ncomms3497
PMID 24084941
PMC PMC3806342
MeSH Animals Cornea / pathology Dry Eye Syndromes / pathology Dry Eye Syndromes / surgery Dry Eye Syndromes / therapy* Embryo, Mammalian Embryonic Stem Cells / cytology* Epithelial Cells / pathology Graft Survival Harderian Gland / transplantation* Lacrimal Apparatus / pathology Lacrimal Apparatus / surgery* Mice Mice, Inbred C57BL Polyglycolic Acid / chemistry Recovery of Function* Regeneration* Tears / metabolism Tissue Engineering Transplantation, Homologous
IF 12.121
Times Cited 67
Mice RBRC00267