RRC ID 2799
Author Tate Y, Yoshiba K, Yoshiba N, Iwaku M, Okiji T, Ohshima H.
Title Odontoblast responses to GaAlAs laser irradiation in rat molars: an experimental study using heat-shock protein-25 immunohistochemistry.
Journal Eur. J. Oral Sci.
Abstract Pulpal responses to gallium-aluminum-arsenide (GaAlAs) laser irradiation applied to the tooth remains to be elucidated. This study aimed to evaluate the effect of the GaAlAs laser on odontoblasts using immunohistochemistry for heat-shock protein (HSP)-25, which labels mature and newly differentiated odontoblasts. The mesial surface of the upper right first molar of 8-wk-old Wistar rats was lased at an output power of 0.5-1.5 W for 180 s. The animals were perfusion-fixed at intervals of 6 h to 30 d after irradiation. At 6 h to 7 d, the intensity of HSP-25-immunoreactivity was found to be disturbed in the coronal odontoblast-layer in an energy-dependent manner. At 30 d, tertiary dentin with/without bone-like tissue was formed abundantly in the dental pulp. Statistical analysis revealed that the area occupied by the new hard tissues was significantly wider in 1.5 W-lased specimens than in 0.5 W-lased specimens. An intense HSP-25 immunoreactivity was seen in the odontoblasts underlying the tertiary dentin, whereas immunoreactivity was weak around the bone-like tissue. It was concluded that the GaAlAs laser may induce the formation of tertiary dentin by influencing the secretory activity of odontoblasts. However, higher energies may cause irreversible changes to the pulp, often leading to the formation of an intrapulpal bone-like tissue.
Volume 114(1)
Pages 50-7
Published 2006-2
DOI 10.1111/j.1600-0722.2006.00261.x
PMID 16460341
MeSH Animals Dental Pulp / injuries* Dental Pulp / metabolism Dentin, Secondary / growth & development* Dentin, Secondary / metabolism HSP27 Heat-Shock Proteins Heat-Shock Proteins / analysis Heat-Shock Proteins / metabolism Immunoenzyme Techniques Lasers / adverse effects* Neoplasm Proteins / analysis Neoplasm Proteins / metabolism Odontoblasts / metabolism Odontoblasts / pathology* Rats Rats, Wistar
IF 1.81
Times Cited 29