| RRC ID |
2799
|
| 著者 |
Tate Y, Yoshiba K, Yoshiba N, Iwaku M, Okiji T, Ohshima H.
|
| タイトル |
Odontoblast responses to GaAlAs laser irradiation in rat molars: an experimental study using heat-shock protein-25 immunohistochemistry.
|
| ジャーナル |
Eur J Oral Sci
|
| Abstract |
Pulpal responses to gallium-aluminum-arsenide (GaAlAs) laser irradiation applied to the tooth remains to be elucidated. This study aimed to evaluate the effect of the GaAlAs laser on odontoblasts using immunohistochemistry for heat-shock protein (HSP)-25, which labels mature and newly differentiated odontoblasts. The mesial surface of the upper right first molar of 8-wk-old Wistar rats was lased at an output power of 0.5-1.5 W for 180 s. The animals were perfusion-fixed at intervals of 6 h to 30 d after irradiation. At 6 h to 7 d, the intensity of HSP-25-immunoreactivity was found to be disturbed in the coronal odontoblast-layer in an energy-dependent manner. At 30 d, tertiary dentin with/without bone-like tissue was formed abundantly in the dental pulp. Statistical analysis revealed that the area occupied by the new hard tissues was significantly wider in 1.5 W-lased specimens than in 0.5 W-lased specimens. An intense HSP-25 immunoreactivity was seen in the odontoblasts underlying the tertiary dentin, whereas immunoreactivity was weak around the bone-like tissue. It was concluded that the GaAlAs laser may induce the formation of tertiary dentin by influencing the secretory activity of odontoblasts. However, higher energies may cause irreversible changes to the pulp, often leading to the formation of an intrapulpal bone-like tissue.
|
| 巻・号 |
114(1)
|
| ページ |
50-7
|
| 公開日 |
2006-2-1
|
| DOI |
10.1111/j.1600-0722.2006.00261.x
|
| PII |
EOS261
|
| PMID |
16460341
|
| MeSH |
Animals
Dental Pulp / injuries*
Dental Pulp / metabolism
Dentin, Secondary / growth & development*
Dentin, Secondary / metabolism
HSP27 Heat-Shock Proteins
Heat-Shock Proteins / analysis
Heat-Shock Proteins / metabolism
Immunoenzyme Techniques
Lasers / adverse effects*
Neoplasm Proteins / analysis
Neoplasm Proteins / metabolism
Odontoblasts / metabolism
Odontoblasts / pathology*
Rats
Rats, Wistar
|
| IF |
2.22
|
| 引用数 |
36
|
|
WOS 分野
|
DENTISTRY, ORAL SURGERY & MEDICINE
|
| リソース情報 |
| ラット |
|
