RRC ID 28
Author Amano T, Matsubayashi H, Ozkan ED, Sasa M, Serikawa T, Ueda T.
Title Aberrant reduction of an inhibitory protein factor in a rat epileptic model.
Journal Epilepsy Res.
Abstract Certain forms of seizure involve excessive glutamate transmission. We have recently identified a protein, referred to as the inhibitory protein factor (IPF), which potently inhibits glutamate uptake into isolated synaptic vesicles. In an effort to understand the mechanism underlying excessive glutamate transmission associated with seizure, we have analyzed IPF content in various brain regions of the spontaneously epileptic rat, SER (tm/tm, zi/zi), the absence-seizure tremor rat, TM (tm/tm), and the seizure-free control rats zitter ZI (zi/zi) and Wistar tremor control, each at 13 weeks of age. IPF content was found to be markedly reduced in the hippocampus, but not in the other brain regions, of SER, compared to the control and TM rats. TM rats also exhibited reduced IPF content compared to seizure-free controls. These changes appear developmentally regulated; no such alteration was observed in 8-week-old rats, which rarely show seizure. These observations indicate that an aberrant decrease in IPF is associated with certain forms of seizure; this decrease could lead to an abnormal increase in the amount of exocytotically released glutamate through its excessive accumulation in synaptic vesicles.
Volume 51(1-2)
Pages 81-91
Published 2002-9
PII S0920121102001225
PMID 12350384
MeSH Aging Animals Antibodies Blotting, Western Brain / anatomy & histology Brain / metabolism* Brain Chemistry Carrier Proteins / metabolism Cytosol / drug effects Cytosol / metabolism DNA Nucleotidyltransferases / metabolism Disease Models, Animal Epilepsy / metabolism* Microfilament Proteins / metabolism Nerve Tissue Proteins / metabolism* Peptides / chemical synthesis Peptides / metabolism* Peptides / therapeutic use Protein Biosynthesis Proteins / metabolism Rats Rats, Inbred Strains Rats, Mutant Strains Rats, Wistar Synaptosomes / metabolism*
IF 2.491
Times Cited 2
WOS Category CLINICAL NEUROLOGY
Resource
Rats SER/Kyo(strainID=28) TRM/Kyo(strainID=11) WTC/Kyo(strainID=18) ZI/Kyo(strainID=23)