論文 - 詳細
| RRC ID | 28097 |
|---|---|
| 著者 | Shibata T, Sekihara S, Fujikawa T, Miyaji R, Maki K, Ishihara T, Koshiba T, Kawabata S. |
| タイトル | Transglutaminase-catalyzed protein-protein cross-linking suppresses the activity of the NF-κB-like transcription factor relish. |
| ジャーナル | Sci Signal |
| Abstract |
Cross-linking of proteins by mammalian transglutaminases (TGs) plays important roles in physiological phenomena such as blood coagulation and skin formation. We show that Drosophila TG suppressed innate immune signaling in the gut. RNA interference (RNAi) directed against TG reduced the life span of flies reared under conventional nonsterile conditions but not of those raised under germ-free conditions. In conventionally reared flies, TG RNAi enhanced the expression of genes encoding antimicrobial peptides in the immune deficiency (IMD) pathway. Wild-type flies that ingested gut lysates prepared from conventionally reared TG RNAi-treated flies had shorter life spans. In conventionally reared flies, TG RNAi triggered apoptosis in the gut and induced the nuclear translocation of Relish, the NF-κB (nuclear factor κB)-like transcription factor of the IMD pathway. Wild-type flies that ingested synthetic amine donors, which inhibit the TG-catalyzed protein-protein cross-linking reaction, showed nuclear translocation of Relish and enhanced expression of genes encoding IMD-controlled antimicrobial peptide genes in the gut. We conclude that TG-catalyzed Relish cross-linking suppressed the IMD signaling pathway to enable immune tolerance against commensal microbes. |
| 巻・号 | 6(285) |
| ページ | ra61 |
| 公開日 | 2013-7-23 |
| DOI | 10.1126/scisignal.2003970 |
| PII | 6/285/ra61 |
| PMID | 23882120 |
| MeSH | Active Transport, Cell Nucleus Animals Antimicrobial Cationic Peptides / chemistry Apoptosis Catalysis Cross-Linking Reagents / chemistry* Disease Models, Animal Drosophila Proteins / metabolism* Drosophila melanogaster Gene Expression Regulation, Enzymologic* Genotype Intestines / microbiology Microscopy, Confocal NF-kappa B / metabolism Protein Interaction Mapping RNA Interference Sepsis / immunology Signal Transduction Subcellular Fractions Transcription Factors / metabolism* Transglutaminases / metabolism* |
| IF | 6.467 |
| 引用数 | 22 |
| WOS 分野 | BIOCHEMISTRY & MOLECULAR BIOLOGY CELL BIOLOGY |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 最多言及媒体 | X(Twitter) |
| 各媒体での言及数の合計 | 8 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| ショウジョウバエ | 7356R-1 7356R-2 |