RRC ID |
28106
|
著者 |
Goss CA, Brockmann DJ, Bushoven JT, Roberts AW.
|
タイトル |
A CELLULOSE SYNTHASE (CESA) gene essential for gametophore morphogenesis in the moss Physcomitrella patens.
|
ジャーナル |
Planta
|
Abstract |
In seed plants, different groups of orthologous genes encode the CELLULOSE SYNTHASE (CESA) proteins that are responsible for cellulose biosynthesis in primary and secondary cell walls. The seven CESA sequences of the moss Physcomitrella patens (Hedw.) B. S. G. form a monophyletic sister group to seed plant CESAs, consistent with independent CESA diversification and specialization in moss and seed plant lines. The role of PpCESA5 in the development of P. patens was investigated by targeted mutagenesis. The cesa5 knockout lines were tested for cellulose deficiency using carbohydrate-binding module affinity cytochemistry and the morphology of the leafy gametophores was analyzed by 3D reconstruction of confocal images. No defects were identified in the development of the filamentous protonema or in production of bud initials that normally give rise to the leafy gametophores. However, the gametophore buds were cellulose deficient and defects in subsequent cell expansion, cytokinesis, and leaf initiation resulted in the formation of irregular cell clumps instead of leafy shoots. Analysis of the cesa5 knockout phenotype indicates that a biophysical model of organogenesis can be extended to the moss gametophore shoot apical meristem.
|
巻・号 |
235(6)
|
ページ |
1355-67
|
公開日 |
2012-6-1
|
DOI |
10.1007/s00425-011-1579-5
|
PMID |
22215046
|
MeSH |
Bryopsida / enzymology*
Bryopsida / genetics*
Bryopsida / growth & development
Cellulose / metabolism
Gene Expression Regulation, Enzymologic
Gene Expression Regulation, Plant
Gene Knockout Techniques
Genes, Essential / genetics*
Genes, Plant / genetics*
Genetic Complementation Test
Genetic Vectors / genetics
Genotype
Germ Cells, Plant / cytology
Germ Cells, Plant / growth & development*
Germ Cells, Plant / metabolism
Glucosyltransferases / genetics*
Glucosyltransferases / metabolism
Microscopy, Confocal
Morphogenesis / genetics*
Reproducibility of Results
Restriction Mapping
Reverse Transcriptase Polymerase Chain Reaction
Transformation, Genetic
|
IF |
3.39
|
引用数 |
20
|
WOS 分野
|
PLANT SCIENCES
|
リソース情報 |
シロイヌナズナ / 植物培養細胞・遺伝子 |
pdp24095 |