RRC ID |
28119
|
Author |
Shinya T, Motoyama N, Ikeda A, Wada M, Kamiya K, Hayafune M, Kaku H, Shibuya N.
|
Title |
Functional characterization of CEBiP and CERK1 homologs in arabidopsis and rice reveals the presence of different chitin receptor systems in plants.
|
Journal |
Plant Cell Physiol
|
Abstract |
Chitin is a representative microbe-associated molecular pattern (MAMP) molecule for various fungi and induces immune responses in many plant species. It has been clarified that the chitin signaling in rice requires a receptor kinase OsCERK1 and a receptor-like protein (Os)CEBiP, which specifically binds chitin oligosaccharides. On the other hand, Arabidopsis requires a receptor kinase (At)CERK1 for chitin signaling but it is not clear whether the plant also requires a CEBiP-like molecule for chitin perception/signaling. To clarify the similarity/difference of the chitin receptor in these two model plants, we first characterized CEBiP homologs in Arabidopsis. Only one of three CEBiP homologs, AtCEBiP (LYM2), showed a high-affinity binding for chitin oligosaccharides similar to rice CEBiP. AtCEBiP also represented the major chitin-binding protein in the Arabidopsis membrane. However, the single/triple knockout (KO) mutants of Arabidopsis CEBiP homologs and the overexpressor of AtCEBiP showed chitin-induced defense responses similar to wild-type Arabidopsis, indicating that AtCEBiP is biochemically functional as a chitin-binding protein but does not contribute to signaling. Studies of the chitin binding properties of the ectodomains of At/OsCERK1 and the chimeric receptors consisting of ecto/cytosolic domains of these molecules indicated that AtCERK1 is sufficient for chitin perception by itself.
|
Volume |
53(10)
|
Pages |
1696-706
|
Published |
2012-10-1
|
DOI |
10.1093/pcp/pcs113
|
PII |
pcs113
|
PMID |
22891159
|
MeSH |
Arabidopsis / immunology
Arabidopsis / metabolism*
Arabidopsis / microbiology
Arabidopsis Proteins / genetics
Arabidopsis Proteins / metabolism*
Chitin / metabolism*
Cytosol / metabolism
Gene Expression Regulation, Plant
Gene Knockout Techniques
Oryza / immunology
Oryza / metabolism*
Oryza / microbiology
Plant Proteins / metabolism*
Plants, Genetically Modified
Protein Serine-Threonine Kinases / metabolism*
Protein Structure, Tertiary
Receptors, Cell Surface / genetics
Receptors, Cell Surface / metabolism*
Signal Transduction
Tobacco / genetics
Tobacco / metabolism
|
IF |
4.062
|
Times Cited |
101
|
WOS Category
|
PLANT SCIENCES
CELL BIOLOGY
|
Resource |
Arabidopsis / Cultured plant cells, genes |
sja16500 |