RRC ID 28145
Author Yano R, Takebayashi Y, Nambara E, Kamiya Y, Seo M.
Title Combining association mapping and transcriptomics identify HD2B histone deacetylase as a genetic factor associated with seed dormancy in Arabidopsis thaliana.
Journal Plant J.
Abstract Seed dormancy is an important adaptive trait that enables germination at the proper time, thereby ensuring plant survival after germination. In Arabidopsis, considerable variation exists in the degree of seed dormancy among wild-type accessions (ecotypes). In this paper, we identify a plant-specific HD2 histone deacetylase gene, HD2B (At5g22650), as a genetic factor associated with seed dormancy. First, genome-wide association mapping of 113 accessions was used to identify single nucleotide polymorphisms that possibly explain natural variation for seed dormancy. Integration of genome-wide association mapping and transcriptome analysis during cold-induced dormancy cycling identified HD2B as the most plausible candidate gene, and quantitative RT-PCR analysis demonstrated that HD2B expression was up-regulated by cold and after-ripening (dry storage of mature seed), treatments that are known to break seed dormancy. Interestingly, quantitative RT-PCR analysis in 106 accessions revealed that the expression of HD2B in imbibed seeds was significantly suppressed in most of the dormant accessions compared with less-dormant accessions, suggesting that suppression of HD2B expression may be important to maintain seed dormancy in dormant accessions. In addition, transgenic seeds of a dormant Cvi-0 accession that carried a 2.5 kb genomic DNA fragment of HD2B cloned from a less-dormant Col-0 accession ((Col)HD2B/Cvi-0) exhibited reduced seed dormancy accompanied by enhanced expression of HD2B when after-ripened or cold-imbibed. Endogenous levels of gibberellin were found to be increased in the imbibed seeds of after-ripened (Col)HD2B/Cvi-0 compared with wild-type Cvi-0. These results suggest that HD2B plays a role in seed dormancy and/or germinability in Arabidopsis thaliana.
Volume 74(5)
Pages 815-28
Published 2013-6
DOI 10.1111/tpj.12167
PMID 23464703
MeSH Arabidopsis / classification Arabidopsis / genetics* Arabidopsis / growth & development Arabidopsis Proteins / genetics* Chromosome Mapping Chromosomes, Plant / genetics Cold Temperature Ecotype Gene Expression Profiling* Gene Expression Regulation, Developmental Gene Expression Regulation, Plant Genome, Plant / genetics Germination / genetics Gibberellins / metabolism Histone Deacetylases / genetics* Plant Dormancy / genetics* Plants, Genetically Modified Polymorphism, Single Nucleotide Reverse Transcriptase Polymerase Chain Reaction Seeds / genetics* Seeds / growth & development Species Specificity
IF 5.726
Times Cited 22
WOS Category PLANT SCIENCES
Resource
Arabidopsis / Cultured plant cells, genes sja