Reference - Detail
| RRC ID | 281 |
|---|---|
| Author | Leulier F, Vidal S, Saigo K, Ueda R, Lemaitre B. |
| Title | Inducible expression of double-stranded RNA reveals a role for dFADD in the regulation of the antibacterial response in Drosophila adults. |
| Journal | Curr Biol |
| Abstract |
In Drosophila, the immune deficiency (Imd) pathway controls antibacterial peptide gene expression in the fat body in response to Gram-negative bacterial infection. The ultimate target of the Imd pathway is Relish, a transactivator related to mammalian P105 and P100 NF-kappaB precursors. Relish is processed in order to translocate to the nucleus, and this cleavage is dependent on both Dredd, an apical caspase related to caspase-8 of mammals, and the fly Ikappa-B kinase complex (dmIKK). dTAK1, a MAPKKK, functions upstream of the dmIKK complex and downstream of Imd, a protein with a death domain similar to that of mammalian receptor interacting protein (RIP). Finally, the peptidoglycan recognition protein-LC (PGRP-LC) acts upstream of Imd and probably functions as a receptor for the Imd pathway. Using inducible expression of dFADD double-stranded RNA, we demonstrate that dFADD is a novel component of the Imd pathway: dFADD double-stranded RNA expression reduces the induction of antibacterial peptide-encoding genes after infection and renders the fly susceptible to Gram-negative bacterial infection. Epistatic studies indicate that dFADD acts between Imd and Dredd. Our results reinforce the parallels between the Imd and the TNF-R1 pathways. |
| Volume | 12(12) |
| Pages | 996-1000 |
| Published | 2002-6-25 |
| DOI | 10.1016/s0960-9822(02)00873-4 |
| PII | S0960-9822(02)00873-4 |
| PMID | 12123572 |
| MeSH | Adaptor Proteins, Signal Transducing* Animals Animals, Genetically Modified Carrier Proteins / genetics Carrier Proteins / physiology* Drosophila / genetics Drosophila / immunology Drosophila / microbiology* Drosophila Proteins / genetics Drosophila Proteins / physiology Fas-Associated Death Domain Protein Female Gene Expression Regulation Gram-Negative Bacteria / physiology Insect Proteins / genetics Male RNA, Double-Stranded / biosynthesis |
| IF | 9.601 |
| Times Cited | 136 |
| WOS Category | BIOCHEMISTRY & MOLECULAR BIOLOGY CELL BIOLOGY |
| Altmetric score |
オルトメトリクス指標項目
|
| Total number of mentions | 0 |
| Resource | |
| Drosophila | 1388R-1 1388R-2 12297R-1 12297R-2 GFP-IR-1 |