論文 - 詳細
| RRC ID | 28400 |
|---|---|
| 著者 | Ward CJ, Beattie KA, Lee EY, Codd GA. |
| タイトル | Colorimetric protein phosphatase inhibition assay of laboratory strains and natural blooms of cyanobacteria: comparisons with high-performance liquid chromatographic analysis for microcystins. |
| ジャーナル | FEMS Microbiol Lett |
| Abstract |
Microcystins are cyclic heptapeptide hepatotoxins commonly produced by bloom-forming genera of cyanobacteria. These toxins are potent and specific inhibitors of protein phosphatases 1 and 2A. We have optimised a rapid, simple and sensitive colorimetric protein phosphatase 1 inhibition assay, utilising the activity of protein phosphatase 1 as expressed in a recombinant strain of Escherichia coli, towards the chromogenic substrate, p-nitrophenyl phosphate. A standard curve for the inhibition of protein phosphatase 1 by microcystin-LR was constructed with an IC50 of about 38 ng ml-1 and a limit of detection of 10-20 ng ml-1. Twenty-three laboratory-grown strains and 25 natural bloom samples of cyanobacteria were analysed by high-performance liquid chromatography for microcystins and by the protein phosphatase 1 inhibition assay. Agreement for the microcystin contents of the samples detected by high-performance liquid chromatography and the protein phosphatase 1 inhibition assay showed good correlation (R2 > 0.93, P < 0.0001). The suitability of the colorimetric protein phosphatase 1 inhibition assay as a screen for cyanobacterial microcystins is discussed. |
| 巻・号 | 153(2) |
| ページ | 465-73 |
| 公開日 | 1997-8-15 |
| DOI | 10.1016/s0378-1097(97)00290-5 |
| PII | S0378-1097(97)00290-5 |
| PMID | 9271876 |
| MeSH | Chromatography, High Pressure Liquid / methods* Colorimetry / methods* Cyanobacteria / chemistry* Enzyme Inhibitors / pharmacology Escherichia coli / genetics Indicators and Reagents Methanol Microcystins Nitrophenols Organophosphorus Compounds Peptides, Cyclic / analysis* Peptides, Cyclic / pharmacology Phosphoprotein Phosphatases / antagonists & inhibitors* Protein Phosphatase 1 Sensitivity and Specificity Solvents |
| IF | 1.987 |
| 引用数 | 120 |
| WOS 分野 | MICROBIOLOGY |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 最多言及媒体 | Patent(IFI CLAIMS) |
| 各媒体での言及数の合計 | 2 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| 藻類 | NIES-42 NIES-103 NIES-104 NIES-595 NIES-298 NIES-102 |