RRC ID 28416
著者 Guillou L, Moon-Van Der Staay SY, Claustre H, Partensky F, Vaulot D.
タイトル Diversity and abundance of Bolidophyceae (Heterokonta) in two oceanic regions.
ジャーナル Appl Environ Microbiol
Abstract The diversity and abundance of the Bolidophyceae (Heterokonta), a newly described picoplanktonic algal class which is a sister group to the diatoms, was assessed in the equatorial Pacific Ocean and in the Mediterranean Sea by culture isolation, molecular biology techniques, and pigment analyses. Eight strains of Bolidophyceae were isolated in culture from different mesotrophic and oligotrophic areas. The corresponding small subunit (SSU) rRNA gene sequences allowed us to design two probes specific for the Bolidophyceae. These probes have been used in natural samples (i) to selectively amplify and detect Bolidophyceae sequences and (ii) to quantify the relative abundance of Bolidophyceae within the picoeukaryote community. Sequences available to date indicate that the class Bolidophyceae comprises at least three different clades, two corresponding to the previously described species Bolidomonas pacifica and Bolidomonas mediterranea and the third one corresponding to a subspecies of B. pacifica. Amplification of the SSU rRNA gene from natural samples with universal primers and hybridization using a Bolidomonas-specific probe followed by a eukaryote-specific probe allowed us to estimate the contribution of the Bolidophyceae to the eukaryotic DNA in both Pacific and Mediterranean waters to be lower than 1%. Similarly, high-performance liquid chromatography analyses of fucoxanthin, the major carotenoid present in Bolidophyceae, indicated that less than 4% of the total chlorophyll a in the picoplanktonic fraction in the equatorial Pacific was due to Bolidophyceae. Consequently, although strains of Bolidophyceae have been isolated from samples collected at several stations, this new class seems to have been a minor component of the natural picoeukaryotic populations in the ecosystems investigated, at least during the periods sampled.
巻・号 65(10)
ページ 4528-36
公開日 1999-10-1
DOI 10.1128/AEM.65.10.4528-4536.1999
PMID 10508085
PMC PMC91603
MeSH Animals Base Sequence DNA, Ribosomal / chemistry Eukaryota / classification Eukaryota / isolation & purification* Molecular Sequence Data Phylogeny Pigments, Biological / analysis Plankton / classification Plankton / isolation & purification*
IF 4.016
引用数 66
WOS 分野 BIOTECHNOLOGY & APPLIED MICROBIOLOGY MICROBIOLOGY
リソース情報
藻類 NIES-2586 NIES-2589 NIES-2682 NIES-2691