RRC ID |
28416
|
著者 |
Guillou L, Moon-Van Der Staay SY, Claustre H, Partensky F, Vaulot D.
|
タイトル |
Diversity and abundance of Bolidophyceae (Heterokonta) in two oceanic regions.
|
ジャーナル |
Appl Environ Microbiol
|
Abstract |
The diversity and abundance of the Bolidophyceae (Heterokonta), a newly described picoplanktonic algal class which is a sister group to the diatoms, was assessed in the equatorial Pacific Ocean and in the Mediterranean Sea by culture isolation, molecular biology techniques, and pigment analyses. Eight strains of Bolidophyceae were isolated in culture from different mesotrophic and oligotrophic areas. The corresponding small subunit (SSU) rRNA gene sequences allowed us to design two probes specific for the Bolidophyceae. These probes have been used in natural samples (i) to selectively amplify and detect Bolidophyceae sequences and (ii) to quantify the relative abundance of Bolidophyceae within the picoeukaryote community. Sequences available to date indicate that the class Bolidophyceae comprises at least three different clades, two corresponding to the previously described species Bolidomonas pacifica and Bolidomonas mediterranea and the third one corresponding to a subspecies of B. pacifica. Amplification of the SSU rRNA gene from natural samples with universal primers and hybridization using a Bolidomonas-specific probe followed by a eukaryote-specific probe allowed us to estimate the contribution of the Bolidophyceae to the eukaryotic DNA in both Pacific and Mediterranean waters to be lower than 1%. Similarly, high-performance liquid chromatography analyses of fucoxanthin, the major carotenoid present in Bolidophyceae, indicated that less than 4% of the total chlorophyll a in the picoplanktonic fraction in the equatorial Pacific was due to Bolidophyceae. Consequently, although strains of Bolidophyceae have been isolated from samples collected at several stations, this new class seems to have been a minor component of the natural picoeukaryotic populations in the ecosystems investigated, at least during the periods sampled.
|
巻・号 |
65(10)
|
ページ |
4528-36
|
公開日 |
1999-10-1
|
DOI |
10.1128/AEM.65.10.4528-4536.1999
|
PMID |
10508085
|
PMC |
PMC91603
|
MeSH |
Animals
Base Sequence
DNA, Ribosomal / chemistry
Eukaryota / classification
Eukaryota / isolation & purification*
Molecular Sequence Data
Phylogeny
Pigments, Biological / analysis
Plankton / classification
Plankton / isolation & purification*
|
IF |
4.016
|
引用数 |
66
|
WOS 分野
|
BIOTECHNOLOGY & APPLIED MICROBIOLOGY
MICROBIOLOGY
|
リソース情報 |
藻類 |
NIES-2586
NIES-2589
NIES-2682
NIES-2691 |