Reference - Detail
| RRC ID | 28443 |
|---|---|
| Author | Nagafuchi A, Shirayoshi Y, Okazaki K, Yasuda K, Takeichi M. |
| Title | Transformation of cell adhesion properties by exogenously introduced E-cadherin cDNA. |
| Journal | Nature |
| Abstract |
E-cadherin is a cell surface glycoprotein responsible for Ca2+-dependent intercellular adhesion between epithelial cells; it is also called uvomorulin, L-CAM (ref. 3), cell-CAM 120/80 (ref.4) or Arc-1 (ref. 5). Because blocking the action of E-cadherin by monoclonal antibodies causes dispersion of compact cell colonies, this molecule is thought to be an important factor for maintenance of multicellular systems. To demonstrate directly that E-cadherin is involved in cell-cell adhesion, we cloned full-length cDNA encoding E-cadherin from F9 cells and introduced it into L fibroblasts deficient in E-cadherin. These L cells acquire strong Ca2+-dependent aggregating activity by expressing the E-cadherin derived from the introduced cDNA and were morphologically transformed so as to form colonies in which cells were tightly connected to each other. |
| Volume | 329(6137) |
| Pages | 341-3 |
| Published | 1987-9-24 |
| DOI | 10.1038/329341a0 |
| PMID | 3498123 |
| MeSH | Amino Acid Sequence Animals Base Sequence Cadherins Calcium / pharmacology Cell Adhesion Cell Line DNA / genetics* DNA, Recombinant Glycoproteins / genetics Glycoproteins / physiology* L Cells / cytology Mice RNA, Messenger / genetics Teratoma Transfection Transformation, Genetic* |
| IF | 42.779 |
| Times Cited | 670 |
| WOS Category | BIOCHEMISTRY & MOLECULAR BIOLOGY |
| Altmetric score |
オルトメトリクス指標項目
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| The most frequently cited source | Patent(IFI CLAIMS) |
| Total number of mentions | 21 |
| Altmetric score changes over past 6months | 0.0 |
| Resource | |
| DNA material | Mouse E-cadherin full length cDNA (RDB01184). |