| RRC ID |
28443
|
| Author |
Nagafuchi A, Shirayoshi Y, Okazaki K, Yasuda K, Takeichi M.
|
| Title |
Transformation of cell adhesion properties by exogenously introduced E-cadherin cDNA.
|
| Journal |
Nature
|
| Abstract |
E-cadherin is a cell surface glycoprotein responsible for Ca2+-dependent intercellular adhesion between epithelial cells; it is also called uvomorulin, L-CAM (ref. 3), cell-CAM 120/80 (ref.4) or Arc-1 (ref. 5). Because blocking the action of E-cadherin by monoclonal antibodies causes dispersion of compact cell colonies, this molecule is thought to be an important factor for maintenance of multicellular systems. To demonstrate directly that E-cadherin is involved in cell-cell adhesion, we cloned full-length cDNA encoding E-cadherin from F9 cells and introduced it into L fibroblasts deficient in E-cadherin. These L cells acquire strong Ca2+-dependent aggregating activity by expressing the E-cadherin derived from the introduced cDNA and were morphologically transformed so as to form colonies in which cells were tightly connected to each other.
|
| Volume |
329(6137)
|
| Pages |
341-3
|
| Published |
1987-9-24
|
| DOI |
10.1038/329341a0
|
| PMID |
3498123
|
| MeSH |
Amino Acid Sequence
Animals
Base Sequence
Cadherins
Calcium / pharmacology
Cell Adhesion
Cell Line
DNA / genetics*
DNA, Recombinant
Glycoproteins / genetics
Glycoproteins / physiology*
L Cells / cytology
Mice
RNA, Messenger / genetics
Teratoma
Transfection
Transformation, Genetic*
|
| IF |
42.779
|
| Times Cited |
670
|
|
WOS Category
|
BIOCHEMISTRY & MOLECULAR BIOLOGY
|
| Resource |
| DNA material |
Mouse E-cadherin full length cDNA (RDB01184). |
