Reference - Detail
| RRC ID | 28449 |
|---|---|
| Author | Oda H, Uemura T, Shiomi K, Nagafuchi A, Tsukita S, Takeichi M. |
| Title | Identification of a Drosophila homologue of alpha-catenin and its association with the armadillo protein. |
| Journal | J Cell Biol |
| Abstract |
The cadherin cell adhesion system plays a central role in cell-cell adhesion in vertebrates, but its homologues are not identified in the invertebrate. alpha-Catenins are a group of proteins associated with cadherins, and this association is crucial for the cadherins' function. Here, we report the cloning of a Drosophila alpha-catenin gene by low stringent hybridization with a mouse alpha E-catenin probe. Isolated cDNAs encoded a 110-kD protein with 60% identity to mouse alpha E-catenin, and this protein was termed D alpha-catenin. The gene of this protein was located at the chromosome band 80B. Immunostaining analysis using a mAb to D alpha-catenin revealed that it was localized to cell-cell contact sites, expressed throughout development and present in a wide variety of tissues. When this protein was immunoprecipitated from detergent extracts of Drosophila embryos or cell lines, several proteins co-precipitated. These included the armadillo product which was known to be a Drosophila homologue of beta-catenin, another cadherin-associated protein in vertebrates, and a 150-kD glycoprotein. These results strongly suggest that Drosophila has a cell adhesion machinery homologous to the vertebrate cadherin-catenin system. |
| Volume | 121(5) |
| Pages | 1133-40 |
| Published | 1993-6-1 |
| DOI | 10.1083/jcb.121.5.1133 |
| PMID | 8501118 |
| PMC | PMC2119693 |
| MeSH | Amino Acid Sequence Animals Armadillo Domain Proteins Base Sequence Cell Adhesion Cell Adhesion Molecules / genetics* Cell Adhesion Molecules / metabolism Chromosome Mapping Cloning, Molecular Cytoskeletal Proteins / genetics* DNA / genetics Drosophila Proteins* Drosophila melanogaster / genetics* Genes, Insect* In Situ Hybridization Molecular Sequence Data Protein Binding Proteins / genetics* Proteins / metabolism Sequence Alignment Trans-Activators* Transcription Factors Vinculin / chemistry alpha Catenin |
| IF | 8.811 |
| Times Cited | 138 |
| WOS Category | CELL BIOLOGY |
| Altmetric score |
オルトメトリクス指標項目
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| Total number of mentions | 0 |
| Resource | |
| DNA material | pBS-DCAP-EK (RDB01294). |