Reference - Detail
RRC ID | 28595 |
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Author | Carr SB, Mecia LB, Phillips SE, Thomas CD. |
Title | Identification, characterization and preliminary X-ray diffraction analysis of the rolling-circle replication initiator protein from plasmid pSTK1. |
Journal | Acta Crystallogr Sect F Struct Biol Cryst Commun |
Abstract |
Antibiotic resistance in bacterial pathogens poses an ever-increasing risk to human health. In antibiotic-resistant strains of Staphylococcus aureus this resistance often resides in extra-chromosomal plasmids, such as those of the pT181 family, which replicate via a rolling-circle mechanism mediated by a plasmid-encoded replication initiation protein. Currently, there is no structural information available for the pT181-family Rep proteins. Here, the crystallization of a catalytically active fragment of a homologous replication initiation protein from the thermophile Geobacillus stearothermophilus responsible for the replication of plasmid pSTK1 is reported. Crystals of the RepSTK1 fragment diffracted to a resolution of 2.5 Å and belonged to space group P2₁2₁2₁. |
Volume | 69(Pt 10) |
Pages | 1123-6 |
Published | 2013-10-1 |
DOI | 10.1107/S1744309113023828 |
PII | S1744309113023828 |
PMID | 24100563 |
PMC | PMC3792671 |
MeSH | DNA Helicases / chemistry* Electrophoresis, Polyacrylamide Gel Geobacillus stearothermophilus / metabolism* Humans Plasmids / metabolism* Trans-Activators / chemistry* X-Ray Diffraction* |
IF | 0.968 |
Times Cited | 1 |
WOS Category | CRYSTALLOGRAPHY BIOPHYSICS BIOCHEMISTRY & MOLECULAR BIOLOGY BIOCHEMICAL RESEARCH METHODS |
Resource | |
DNA material | pSTE33 (RDB00920) |