Reference - Detail
| RRC ID | 285 |
|---|---|
| Author | Takehana A, Katsuyama T, Yano T, Oshima Y, Takada H, Aigaki T, Kurata S. |
| Title | Overexpression of a pattern-recognition receptor, peptidoglycan-recognition protein-LE, activates imd/relish-mediated antibacterial defense and the prophenoloxidase cascade in Drosophila larvae. |
| Journal | Proc Natl Acad Sci U S A |
| Abstract |
In Drosophila, microbial infection activates an antimicrobial defense system involving the activation of proteolytic cascades in the hemolymph and intracellular signaling pathways, the immune deficiency (imd) and Toll pathways, in immune-responsive tissues. The mechanisms for microbial recognition are largely unknown. We report that, in larvae, the imd-mediated antibacterial defense is activated by peptidoglycan-recognition protein (PGRP)-LE, a PGRP-family member in Drosophila. Consistent with this, PGRP-LE binds to the diaminopimelic acid-type peptidoglycan, a cell-wall component of the bacteria capable of activating the imd pathway, but not to the lysine-type peptidoglycan. Moreover, PGRP-LE activates the prophenoloxidase cascade, a proteolytic cascade in the hemolymph. Therefore, PGRP-LE acts as a pattern-recognition receptor to the diaminopimelic acid-type peptidoglycan and activates both the proteolytic cascade and intracellular signaling in Drosophila immunity. |
| Volume | 99(21) |
| Pages | 13705-10 |
| Published | 2002-10-15 |
| DOI | 10.1073/pnas.212301199 |
| PII | 212301199 |
| PMID | 12359879 |
| PMC | PMC129750 |
| MeSH | Animals Base Sequence Carrier Proteins / genetics Carrier Proteins / immunology* Carrier Proteins / metabolism Catechol Oxidase / metabolism* DNA / genetics Drosophila / genetics Drosophila / growth & development Drosophila / immunology* Drosophila / metabolism* Drosophila Proteins / genetics Drosophila Proteins / immunology* Drosophila Proteins / metabolism Enzyme Precursors / metabolism* Gene Expression Genes, Insect Larva / immunology Larva / metabolism Peptidoglycan / metabolism Signal Transduction Transcription Factors / genetics Transcription Factors / immunology* |
| IF | 9.412 |
| Times Cited | 235 |
| WOS Category | BIOCHEMISTRY & MOLECULAR BIOLOGY |
| Resource | |
| Drosophila | |