論文 - 詳細
| RRC ID | 28620 |
|---|---|
| 著者 | Tanaka S, Kodama T, Nonaka T, Toyoda H, Arai M, Fukazawa M, Honda Y, Honda M, Mignot E. |
| タイトル | Transcriptional regulation of the hypocretin/orexin gene by NR6A1. |
| ジャーナル | Biochem Biophys Res Commun |
| Abstract |
The hypocretin (also known as orexin) neuropeptide system coordinates the regulation of various physiological processes. A reduction in Nr6a1 expression was observed in hypocretin neuron-ablated transgenic mice. To show that prepro-hypocretin transcription is functionally modulated by NR6A1, we performed chromatin immunoprecipitation (ChIP) analysis, double-immunostaining, a luciferase reporter assay, and an in utero electroporation study. ChIP analysis showed that endogenous NR6A1 binds to a putative NR6A1-binding site. Double-immunostaining indicated almost all hypocretin neurons were positive for NR6A1 immunoreactivity. NR6A1 overexpression in SH-SY5Y cells modulated hypocretin promoter activity, an effect that was countered by lacking a putative NR6A1-binding site. Electroporation with Nr6a1 in the foetal hypothalamus promoted hypocretin transcription as compared to GFP-electroporation. These experiments confirmed that NR6A1 works as a regulator for hypocretin transcription. |
| 巻・号 | 403(2) |
| ページ | 178-83 |
| 公開日 | 2010-12-10 |
| DOI | 10.1016/j.bbrc.2010.11.001 |
| PII | S0006-291X(10)02033-4 |
| PMID | 21056546 |
| PMC | PMC3012206 |
| MeSH | Animals Base Sequence Cell Line DNA Mutational Analysis Gene Expression Regulation* Humans Hypothalamus / metabolism Immunoprecipitation Intracellular Signaling Peptides and Proteins / genetics* Male Mice Mice, Inbred C57BL Molecular Sequence Data Neuropeptides / genetics* Nuclear Receptor Subfamily 6, Group A, Member 1 / metabolism* Orexins Promoter Regions, Genetic Sequence Deletion Transcription, Genetic |
| IF | 2.985 |
| 引用数 | 14 |
| WOS 分野 | BIOPHYSICS BIOCHEMISTRY & MOLECULAR BIOLOGY |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 最多言及媒体 | Patent(IFI CLAIMS) |
| 各媒体での言及数の合計 | 2 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| 遺伝子材料 | pCAG-mNr6a1 (RDB08376) NR6A1/TNT (RDB08377) |