RRC ID |
28620
|
著者 |
Tanaka S, Kodama T, Nonaka T, Toyoda H, Arai M, Fukazawa M, Honda Y, Honda M, Mignot E.
|
タイトル |
Transcriptional regulation of the hypocretin/orexin gene by NR6A1.
|
ジャーナル |
Biochem Biophys Res Commun
|
Abstract |
The hypocretin (also known as orexin) neuropeptide system coordinates the regulation of various physiological processes. A reduction in Nr6a1 expression was observed in hypocretin neuron-ablated transgenic mice. To show that prepro-hypocretin transcription is functionally modulated by NR6A1, we performed chromatin immunoprecipitation (ChIP) analysis, double-immunostaining, a luciferase reporter assay, and an in utero electroporation study. ChIP analysis showed that endogenous NR6A1 binds to a putative NR6A1-binding site. Double-immunostaining indicated almost all hypocretin neurons were positive for NR6A1 immunoreactivity. NR6A1 overexpression in SH-SY5Y cells modulated hypocretin promoter activity, an effect that was countered by lacking a putative NR6A1-binding site. Electroporation with Nr6a1 in the foetal hypothalamus promoted hypocretin transcription as compared to GFP-electroporation. These experiments confirmed that NR6A1 works as a regulator for hypocretin transcription.
|
巻・号 |
403(2)
|
ページ |
178-83
|
公開日 |
2010-12-10
|
DOI |
10.1016/j.bbrc.2010.11.001
|
PII |
S0006-291X(10)02033-4
|
PMID |
21056546
|
PMC |
PMC3012206
|
MeSH |
Animals
Base Sequence
Cell Line
DNA Mutational Analysis
Gene Expression Regulation*
Humans
Hypothalamus / metabolism
Immunoprecipitation
Intracellular Signaling Peptides and Proteins / genetics*
Male
Mice
Mice, Inbred C57BL
Molecular Sequence Data
Neuropeptides / genetics*
Nuclear Receptor Subfamily 6, Group A, Member 1 / metabolism*
Orexins
Promoter Regions, Genetic
Sequence Deletion
Transcription, Genetic
|
IF |
2.985
|
引用数 |
14
|
WOS 分野
|
BIOPHYSICS
BIOCHEMISTRY & MOLECULAR BIOLOGY
|
リソース情報 |
遺伝子材料 |
pCAG-mNr6a1 (RDB08376)
NR6A1/TNT (RDB08377) |