| RRC ID |
28667
|
| Author |
Nishi N, Itoh A, Fujiyama A, Yoshida N, Araya S, Hirashima M, Shoji H, Nakamura T.
|
| Title |
Development of highly stable galectins: truncation of the linker peptide confers protease-resistance on tandem-repeat type galectins.
|
| Journal |
FEBS Lett
|
| Abstract |
Galectin-9 and galectin-8, members of beta-galactoside-binding animal lectin family, are promising agents for the treatment of immune-related and neoplastic diseases. The proteins consist of two carbohydrate recognition domains joined by a linker peptide, which is highly susceptible to proteolysis. To increase protease resistance, we prepared mutant proteins by serial truncation of the linker peptide. As a result, mutant forms lacking the entire linker peptide were found to be highly stable against proteolysis and retained their biological activities. These mutant proteins might be useful tools for analyzing the biological functions and evaluating the therapeutic potential of galectin-9 and galectin-8.
|
| Volume |
579(10)
|
| Pages |
2058-64
|
| Published |
2005-4-11
|
| DOI |
10.1016/j.febslet.2005.02.054
|
| PII |
S0014-5793(05)00287-5
|
| PMID |
15811318
|
| MeSH |
Base Sequence
DNA, Complementary
Galectins / genetics
Galectins / metabolism*
Peptide Hydrolases / metabolism*
Tandem Repeat Sequences*
|
| IF |
3.057
|
| Times Cited |
102
|
|
WOS Category
|
BIOPHYSICS
BIOCHEMISTRY & MOLECULAR BIOLOGY
CELL BIOLOGY
|
| Resource |
| DNA material |
pET-G9Null-11a (RDB08451)
pET-G9Null R65D-2a (RDB08452)
pET-G9Null R212D-3a (RDB08453)
pGEX-G8Null-1a (RDB08454)
pET-G8Null-5a (RDB08455) |
