| RRC ID |
28699
|
| 著者 |
Tapparo A, Satre M, Klein G.
|
| タイトル |
Cloning, sequencing and developmental expression of the genes encoding S4 and S10 ribosomal proteins in the cellular slime mould Dictyostelium discoideum.
|
| ジャーナル |
Curr Genet
|
| Abstract |
The sequences of the rps4 and rps10 genes encoding the Dictyostelium discoideum homologues of the basic ribosomal proteins S4 and S10 were determined from cDNA and genomic DNA clones. They are expressed respectively as 266 and 153 amino-acid-long proteins. In both cases, the N-terminal methionine is cleaved in the mature proteins. S4 contains two putative nuclear targeting signals and displays a strong overall identity (around 60%) to eukaryotic S4 homologues. The rps10 gene harbours a 314-bp intron located close to its 5'-coding end. The overall identity between D. discoideum S10 and eukaryotic homologues is around 38% and rises to 53% in the N-terminal domain. Southern blots suggest that both S4 and S10 are encoded by single genes that are regulated during development. The corresponding mRNAs decrease sharply after 8 h of differentiation.
|
| 巻・号 |
34(5)
|
| ページ |
410-8
|
| 公開日 |
1998-12-1
|
| DOI |
10.1007/s002940050415
|
| PMID |
9871125
|
| MeSH |
Amino Acid Sequence
Animals
Base Sequence
Blotting, Northern
Cloning, Molecular
DNA, Protozoan / chemistry
DNA, Protozoan / genetics
Dictyostelium / chemistry
Dictyostelium / genetics*
Gene Dosage
Gene Expression Regulation, Developmental
Genes, Protozoan / genetics*
Molecular Sequence Data
Phylogeny
RNA, Messenger / genetics
RNA, Messenger / metabolism
Restriction Mapping
Ribosomal Proteins / chemistry
Ribosomal Proteins / genetics*
Sequence Alignment
Sequence Analysis, DNA
Sequence Homology, Amino Acid
|
| IF |
3.464
|
| 引用数 |
5
|
|
WOS 分野
|
GENETICS & HEREDITY
|
| リソース情報 |
| 細胞性粘菌 |
G01148 |
