RRC ID 28706
Author Insall R, Müller-Taubenberger A, Machesky L, Köhler J, Simmeth E, Atkinson SJ, Weber I, Gerisch G.
Title Dynamics of the Dictyostelium Arp2/3 complex in endocytosis, cytokinesis, and chemotaxis.
Journal Cell Motil Cytoskeleton
Abstract The Arp2/3 complex is a ubiquitous and important regulator of the actin cytoskeleton. Here we identify this complex from Dictyostelium and investigate its dynamics in live cells. The predicted sequences of the subunits show a strong homology to the members of the mammalian complex, with the larger subunits generally better conserved than the smaller ones. In the highly motile cells of Dictyostelium, the Arp2/3 complex is rapidly re-distributed to the cytoskeleton in response to external stimuli. Fusions of Arp3 and p41-Arc with GFP reveal that in phagocytosis, macropinocytosis, and chemotaxis the complex is recruited within seconds to sites where actin polymerization is induced. In contrast, there is little or no localization to the cleavage furrow during cytokinesis. Rather the Arp2/3 complex is enriched in ruffles at the polar regions of mitotic cells, which suggests a role in actin polymerization in these ruffles.
Volume 50(3)
Pages 115-28
Published 2001-11-1
DOI 10.1002/cm.10005
PII 10.1002/cm.10005
PMID 11807934
MeSH Actin-Related Protein 2 Actin-Related Protein 3 Actins / analysis Actins / metabolism* Amino Acid Sequence Animals Cell Division / physiology* Chemotaxis / physiology* Cyclic AMP / pharmacology Cytoskeletal Proteins* Dictyostelium / chemistry* Endocytosis / physiology* Endosomes / metabolism Green Fluorescent Proteins Histocytochemistry / methods Luminescent Proteins / metabolism Macromolecular Substances Molecular Sequence Data Phagocytosis / physiology Pinocytosis Sequence Homology, Amino Acid Signal Transduction Species Specificity
Times Cited 104
Cellular slime molds G02982 G03201 S90289