RRC ID 28708
Author Décavé E, Garrivier D, Bréchet Y, Fourcade B, Bruckert F.
Title Shear flow-induced detachment kinetics of Dictyostelium discoideum cells from solid substrate.
Journal Biophys. J.
Abstract Using Dictyostelium discoideum as a model organism of specific and nonspecific adhesion, we studied the kinetics of shear flow-induced cell detachment. For a given cell, detachment occurs for values of the applied hydrodynamic stress above a threshold. Cells are removed from the substrate with an apparent first-order rate constant that strongly depends on the applied stress. The threshold stress depends on cell size and physicochemical properties of the substrate, but is not affected by depolymerization of the actin and tubulin cytoskeleton. In contrast, the kinetics of cell detachment is almost independent of cell size, but is strongly affected by a modification of the substrate and the presence of an intact actin cytoskeleton. These results are interpreted in the framework of a peeling model. The threshold stress and the cell-detachment rate measure the local equilibrium energy and the dissociation rate constant of the adhesion bridges, respectively.
Volume 82(5)
Pages 2383-95
Published 2002-5
DOI 10.1016/S0006-3495(02)75583-5
PII S0006-3495(02)75583-5
PMID 11964228
PMC PMC1302030
MeSH Animals Cell Adhesion / physiology* Cell Division Cell Size Dictyostelium / cytology Dictyostelium / physiology* Kinetics Mathematics Models, Biological Movement Stress, Mechanical
IF 3.495
Times Cited 50
WOS Category BIOPHYSICS
Resource
Cellular slime molds