RRC ID 28732
Author Nagayama K, Itono S, Yoshida T, Ishiguro S, Ochiai H, Ohmachi T.
Title Antisense RNA inhibition of the beta subunit of the Dictyostelium discoideum mitochondrial processing peptidase induces the expression of mitochondrial proteins.
Journal Biosci Biotechnol Biochem
Abstract We cloned and characterized a cDNA encoding the Dictyostelium discoideum beta subunit of mitochondrial processing peptidase (Ddbeta-MPP). Western blot analysis of the mitochondrial subfractions revealed that Ddbeta-MPP is located in the mitochondrial matrix and membrane, whereas Dd(alpha)-MPP, another subunit of DdMPP, is located only in the matrix. Although expression of Ddbeta-MPP mRNA is down-regulated during early development, the level of the Ddbeta-MPP protein is constant throughout the Dictyostelium life cycle. In a transformant expressing the antisense RNA of the beta-MPP gene, unexpectedly, the beta-MPP protein increased about 1.8-fold relative to the wild type, and its mRNA increased 4.5-fold. Expression of other mitochondrial proteins, alpha-MPP and Cox IV, was also induced. These results suggest that antisense RNA inhibition of the beta-MPP gene induces gene expression of mitochondrial proteins, presumably in a retrograde signaling manner. This is the pathway of the transfer of information from the mitochondria to the nucleus.
Volume 72(7)
Pages 1836-46
Published 2008-7-1
DOI 10.1271/bbb.80106
PII JST.JSTAGE/bbb/80106
PMID 18603769
MeSH Animals Dictyostelium / enzymology* Gene Expression Regulation* Metalloendopeptidases / antagonists & inhibitors* Mitochondrial Proteins / genetics* Protein Subunits RNA, Antisense / physiology* RNA, Messenger / analysis
IF 1.516
Times Cited 14
WOS Category FOOD SCIENCE & TECHNOLOGY CHEMISTRY, APPLIED BIOTECHNOLOGY & APPLIED MICROBIOLOGY BIOCHEMISTRY & MOLECULAR BIOLOGY
Resource
Cellular slime molds G01338