| RRC ID |
28750
|
| Author |
Sano T, Takagi H, Nagano K, Nishikawa M, Kaya K.
|
| Title |
Accurate LC-MS analyses for microcystins using per-15N-labeled microcystins.
|
| Journal |
Anal Bioanal Chem
|
| Abstract |
Per-(15)N-labeled microcystins were prepared for use as surrogates for accurate liquid chromatography-mass spectrometry analysis. Two strains of Microcystis aeruginosa were cultured in (15)NO(3)-containing TS-15 medium. To change from the incorporation of (14)N to (15)N into all cell components, cells of Microcystis aeruginosa were precultured in Na(15)NO(3)-containing medium for more than 6 months. After mass cultivation of the strains, cells of each strain were harvested and lyophilized. Microcystin variants were extracted from the lyophilized cells and per-(15)N-labeled microcystin variants were purified using high-performance liquid chromatography and high-performance thin-layer chromatography. The structures of per-(15)N-labeled microcystin variants were confirmed by their mass spectrometry spectra and NMR spectra. When per-(15)N-labeled microcystins were used as surrogates for quantitative analysis of these toxins in cyanobacterial cells, excellent accuracy (98-106%) was obtained, with the m/z of M(+), [M+1](+), and [M+2](+) of both microcystins and the per-(15)N-labeled microcystins as surrogates being completely separated. In conclusion, per-(15)N-labeled microcystins are excellent surrogates for microcystin analysis using liquid chromatography-mass spectrometry.
|
| Volume |
399(7)
|
| Pages |
2511-6
|
| Published |
2011-3-1
|
| DOI |
10.1007/s00216-010-4639-y
|
| PMID |
21221535
|
| MeSH |
Chromatography, High Pressure Liquid / methods
Chromatography, Thin Layer / methods
Mass Spectrometry
Microcystins / chemistry*
Microcystins / isolation & purification*
Microcystis / chemistry*
Microcystis / metabolism
Nitrogen Isotopes / metabolism
Nuclear Magnetic Resonance, Biomolecular
|
| IF |
3.637
|
| Times Cited |
14
|
|
WOS Category
|
BIOCHEMICAL RESEARCH METHODS
CHEMISTRY, ANALYTICAL
|
| Resource |
| Algae |
NIES-107 |
