RRC ID 28753
Author Kaneko HH, Shimada A, Hirayama K.
Title Short-term algal toxicity test based on phosphate uptake.
Journal Water Res
Abstract In order to develop a short-term algal toxicity test, the growth of and the phosphate uptake by the green alga Selenastrum capricornutum during batch culture were observed. In the control medium, S. capricornutum took up phosphate earlier than it grew. It was also observed that the phosphate uptake was inhibited by the presence of a toxicant. From these results, phosphate uptake was considered as one of the useful effect parameters for a short-term algal toxicity test. As the removal rate of phosphate from the medium is a function of the amount of algal cell initially inoculated, the test period is variable. The relationship between the amount of inoculation and phosphate uptake was examined and the test conditions suitable for a 3-h toxicity test were established as one example. According to this test procedure, the inhibitory effect of some toxicants on the phosphate uptake was determined. For comparison, a conventional algal assay based on algal growth was also performed. The EC50s for both tests were close. This indicated that the algal toxicity test method proposed in this paper would be useful for the uses where rapidity is required.
Volume 38(8)
Pages 2173-7
Published 2004-4-1
DOI 10.1016/j.watres.2004.02.006
PII S0043135404000697
PMID 15087199
MeSH Carbon / chemistry Carbon / pharmacology Chlorophyta / drug effects Chlorophyta / growth & development Chlorophyta / metabolism* Coal Ash Culture Media Dose-Response Relationship, Drug Lethal Dose 50 Metals, Heavy / chemistry Metals, Heavy / pharmacology Particulate Matter Phosphates / metabolism* Toxicity Tests Water Pollutants, Chemical / pharmacology Water Pollutants, Chemical / toxicity
IF 7.913
Times Cited 12
Algae NIES-35