論文 - 詳細
| RRC ID | 28756 |
|---|---|
| 著者 | Kobayashi M. |
| タイトル | In vivo antioxidant role of astaxanthin under oxidative stress in the green alga Haematococcus pluvialis. |
| ジャーナル | Appl Microbiol Biotechnol |
| Abstract |
Intracellular production of active oxygen in the green alga Haematococcus pluvialis was studied by measuring the capacity for in vivo conversion of 2',7'-dichlorohydrofluorescein diacetate to the fluorescent dye dichlorofluorescein in different algal cell types (i.e., vegetative, immature cyst and mature cyst cells). The increase in formation of dichlorofluorescein by methyl viologen (superoxide anion radical generator) was linear for 2 h in immature cyst cells (low astaxanthin) in a methyl viologen-concentration-dependent manner, while no production was detected in mature (high astaxanthin) cysts. Compared to cyst cells, formation of dichlorofluorescein in vegetative cells (no astaxanthin) was markedly increased by methyl viologen. The formation of dichlorofluorescein in cyst cells was decreased with higher astaxanthin content under excessive oxidative stress. All of the active oxygen species tested (singlet oxygen, superoxide anion radical, hydrogen peroxide and peroxy radical) at 10(-3) M increased the intracellular dichlorofluorescein formation in immature cysts, but did not increase the dichlorofluorescein content of mature cysts. Therefore, astaxanthin in cyst cells appeared to function as an antioxidant agent against oxidative stress. |
| 巻・号 | 54(4) |
| ページ | 550-5 |
| 公開日 | 2000-10-1 |
| DOI | 10.1007/s002530000416 |
| PMID | 11092631 |
| MeSH | Antioxidants / analysis* Chlorophyta / metabolism* Fluoresceins / metabolism Oxidative Stress* Xanthophylls beta Carotene / analogs & derivatives* beta Carotene / physiology |
| IF | 3.53 |
| 引用数 | 79 |
| WOS 分野 | BIOTECHNOLOGY & APPLIED MICROBIOLOGY |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 最多言及媒体 | Patent(IFI CLAIMS) |
| 各媒体での言及数の合計 | 3 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| 藻類 | NIES-144 |