Reference - Detail
| RRC ID | 28840 |
|---|---|
| Author | Morawska M, Ulrich HD. |
| Title | An expanded tool kit for the auxin-inducible degron system in budding yeast. |
| Journal | Yeast |
| Abstract |
Fusion of inducible degradation signals, so-called degrons, to cellular proteins is an elegant method of controlling protein levels in vivo. Recently, a degron system relying on the plant hormone auxin has been described for use in yeast and vertebrate cells. We now report the construction of a series of vectors that significantly enhance the versatility of this auxin-inducible degron (AID) system in Saccharomyces cerevisiae. We have minimized the size of the degron and appended a series of additional epitope tags, allowing detection by commercial antibodies or fluorescence microscopy. The vectors are compatible with PCR-based genomic tagging strategies, allow for C- or N-terminal fusion of the degron, and provide a range of selection markers. Application to a series of yeast proteins, including essential replication factors, provides evidence for a general usefulness of the system. |
| Volume | 30(9) |
| Pages | 341-51 |
| Published | 2013-9-1 |
| DOI | 10.1002/yea.2967 |
| PMID | 23836714 |
| PMC | PMC4171812 |
| MeSH | Gene Expression Regulation, Fungal / drug effects* Genetic Vectors Genetics, Microbial / methods* Indoleacetic Acids / metabolism* Molecular Biology / methods* Mycology / methods Saccharomyces cerevisiae / drug effects* Saccharomyces cerevisiae / genetics* Saccharomyces cerevisiae / metabolism Saccharomyces cerevisiae Proteins / biosynthesis Saccharomyces cerevisiae Proteins / genetics |
| IF | 3.143 |
| Times Cited | 120 |
| WOS Category | MYCOLOGY BIOTECHNOLOGY & APPLIED MICROBIOLOGY MICROBIOLOGY BIOCHEMISTRY & MOLECULAR BIOLOGY |
| Altmetric score |
オルトメトリクス指標項目
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| The most frequently cited source | Patent(IFI CLAIMS) |
| Total number of mentions | 5 |
| Altmetric score changes over past 6months | 0.0 |
| Resource | |
| Yeast | BYP6739 BYP6740 BYP6744 BYP6851 BYP6852 |