| RRC ID |
28891
|
| 著者 |
Olesnicky EC, Killian DJ, Garcia E, Morton MC, Rathjen AR, Sola IE, Gavis ER.
|
| タイトル |
Extensive use of RNA-binding proteins in Drosophila sensory neuron dendrite morphogenesis.
|
| ジャーナル |
G3 (Bethesda)
|
| Abstract |
The large number of RNA-binding proteins and translation factors encoded in the Drosophila and other metazoan genomes predicts widespread use of post-transcriptional regulation in cellular and developmental processes. Previous studies identified roles for several RNA-binding proteins in dendrite branching morphogenesis of Drosophila larval sensory neurons. To determine the larger contribution of post-transcriptional gene regulation to neuronal morphogenesis, we conducted an RNA interference screen to identify additional Drosophila proteins annotated as either RNA-binding proteins or translation factors that function in producing the complex dendritic trees of larval class IV dendritic arborization neurons. We identified 88 genes encoding such proteins whose knockdown resulted in aberrant dendritic morphology, including alterations in dendritic branch number, branch length, field size, and patterning of the dendritic tree. In particular, splicing and translation initiation factors were associated with distinct and characteristic phenotypes, suggesting that different morphogenetic events are best controlled at specific steps in post-transcriptional messenger RNA metabolism. Many of the factors identified in the screen have been implicated in controlling the subcellular distributions and translation of maternal messenger RNAs; thus, common post-transcriptional regulatory strategies may be used in neurogenesis and in the generation of asymmetry in the female germline and embryo.
|
| 巻・号 |
4(2)
|
| ページ |
297-306
|
| 公開日 |
2014-2-19
|
| DOI |
10.1534/g3.113.009795
|
| PII |
g3.113.009795
|
| 解説 |
32423R-2,4119R-2,5439R-1,6961R-3,7903R-1,11505R-2,11726R-1,6946R-1,10868R-1,5263R-2
|
| PMID |
24347626
|
| PMC |
PMC3931563
|
| MeSH |
Animals
Dendrites / metabolism*
Drosophila / genetics*
Drosophila / growth & development
Drosophila / metabolism
Drosophila Proteins / genetics*
Drosophila Proteins / metabolism
Larva / cytology
Larva / metabolism
Morphogenesis / genetics*
Neurogenesis / genetics*
Protein Biosynthesis
RNA-Binding Proteins / genetics*
RNA-Binding Proteins / metabolism
Sensory Receptor Cells / cytology
Sensory Receptor Cells / metabolism*
|
| IF |
2.781
|
| 引用数 |
13
|
|
WOS 分野
|
GENETICS & HEREDITY
|
| リソース情報 |
| ショウジョウバエ |
32423R-2
4119R-2
5439R-1
6961R-3
7903R-1
11505R-2
11726R-1
6946R-1
10868R-1
5263R-2 |
