RRC ID 28937
Author Egelhoff TT, Brown SS, Spudich JA.
Title Spatial and temporal control of nonmuscle myosin localization: identification of a domain that is necessary for myosin filament disassembly in vivo.
Journal J. Cell Biol.
Abstract Myosin null mutants of Dictyostelium are defective for cytokinesis, multicellular development, and capping of surface proteins. We have used these cells as transformation recipients for an altered myosin heavy chain gene that encodes a protein bearing a carboxy-terminal 34-kD truncation. This truncation eliminates threonine phosphorylation sites previously shown to control filament assembly in vitro. Despite restoration of growth in suspension, development, and ability to cap cell surface proteins, these delta C34-truncated myosin transformants display severe cytoskeletal abnormalities, including excessive localization of the truncated myosin to the cortical cytoskeleton, impaired cell shaped dynamics, and a temporal defect in myosin dissociation from beneath capped surface proteins. These data demonstrate that the carboxy-terminal domain of myosin plays a critical role in regulating the disassembly of the protein from contractile structures in vivo.
Volume 112(4)
Pages 677-88
Published 1991-2
PMID 1899668
PMC PMC2288861
MeSH Cell Compartmentation Dictyostelium / genetics Dictyostelium / metabolism Fungal Proteins / chemistry Fungal Proteins / genetics Fungal Proteins / metabolism Mutagenesis, Site-Directed Myosins / chemistry* Myosins / genetics Myosins / metabolism* Phosphorylation Structure-Activity Relationship Threonine / metabolism Transfection
IF 8.891
Times Cited 86
Cellular slime molds S00441 S00442